TY - JOUR
T1 - Osteopontin Stabilizes Metastable States Prior to Nucleation during Apatite Formation
AU - Ibsen, Casper Jon Steenberg
AU - Gebauer, Denis
AU - Birkedal, Henrik
PY - 2016/12/13
Y1 - 2016/12/13
N2 - Osteopontin, which is a phosphoprotein with strong ties to in vivo bone mineralization, is shown to change the precipitation pathway of calcium phosphate. We show that the presence of the phosphoprotein, even in minute concentrations, can stabilize an otherwise oversaturated mixture against precipitation. At moderate concentrations, we find that the protein introduces a new intermediate state into the reaction pathway leading to apatite formation. This new intermediate was found to share many characteristics of a coacervate or polymer-induced liquid-like precursor (PILP) phase. Our results show that these types of complex phases should be considered when discussing the mechanisms of bone mineralization on a subcellular level.
AB - Osteopontin, which is a phosphoprotein with strong ties to in vivo bone mineralization, is shown to change the precipitation pathway of calcium phosphate. We show that the presence of the phosphoprotein, even in minute concentrations, can stabilize an otherwise oversaturated mixture against precipitation. At moderate concentrations, we find that the protein introduces a new intermediate state into the reaction pathway leading to apatite formation. This new intermediate was found to share many characteristics of a coacervate or polymer-induced liquid-like precursor (PILP) phase. Our results show that these types of complex phases should be considered when discussing the mechanisms of bone mineralization on a subcellular level.
U2 - 10.1021/acs.chemmater.6b01088
DO - 10.1021/acs.chemmater.6b01088
M3 - Journal article
AN - SCOPUS:85006282651
SN - 0897-4756
VL - 28
SP - 8550
EP - 8555
JO - Chemistry of Materials
JF - Chemistry of Materials
IS - 23
ER -