Osteoblastogenesis Alters Small RNA Profiles in EVs Derived from Bone Marrow Stem Cells (BMSCs) and Adipose Stem Cells (ASCs)

Yan Yan, Clare Chang, Junyi Su, Morten T Venø, Jørgen Kjems*

*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Abstract

Multipotent stem cells (MSCs) are used in various therapeutic applications based on their paracrine secretion activity. Here, we set out to identify and characterize the paracrine factors released during osteoblastogenesis, with a special focus on small non-coding RNAs released in extracellular vesicles (EVs). Bone marrow stem cells (BMSCs) and adipose stem cells (ASCs) from healthy human donors were used as representatives of MSCs. We isolated EVs secreted before and after induction of osteoblastic differentiation and found that the EVs contained a specific subset of microRNAs (miRNAs) and tRNA-derived small RNAs (tsRNA) compared to their parental cells. Osteoblastic differentiation had a larger effect on the small RNA profile of BMSC-EVs relative to ASC-EVs. Our data showed that EVs from different MSC origin exhibited distinct expression profiles of small RNA profiles when undergoing osteoblastogenesis, a factor that should be taken into consideration for stem cell therapy.

Original languageEnglish
Article number387
JournalBiomedicines
Volume8
Issue10
Pages (from-to)1-17
Number of pages17
ISSN2227-9059
DOIs
Publication statusPublished - Sept 2020

Keywords

  • Adipose stem cells
  • Bone marrow stem cell
  • Extracellular vesicles
  • MicroRNA
  • Multipotent stem cell
  • Osteoblastogenesis
  • TRNA-derived small RNA

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