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Origin and removal of mixed-phase artifacts in gradient sensitivity enhanced heteronuclear single quantum correlation spectra

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Here we describe phasing anomalies observed in gradient sensitivity enhanced (15)N-(1)H HSQC spectra, and analyze their origin. It is shown that, as a result of (15)N off-resonance effects, dispersive contributions to the (1)H signal become detectable, and lead to (15)N-offset dependent phase errors. Strategies that effectively suppress these artifacts are presented.
Original languageEnglish
JournalJournal of Biomolecular N M R
Pages (from-to)199-207
Number of pages9
Publication statusPublished - 2011

    Research areas

  • Artifacts, Magnetic Resonance Spectroscopy, Reproducibility of Results

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