Organization of the influenza virus replication machinery

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Arne Möller, National Resource for Automated Molecular Microscopy, Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA., Denmark
  • Robert N Kirchdoerfer, Denmark
  • Clinton S Potter, Denmark
  • Bridget Carragher, Denmark
  • Ian A Wilson, Denmark

Influenza virus ribonucleoprotein complexes (RNPs) are central to the viral life cycle and in adaptation to new host species. RNPs are composed of the viral genome, viral polymerase, and many copies of the viral nucleoprotein. In vitro cell expression of all RNP protein components with four of the eight influenza virus gene segments enabled structural determination of native influenza virus RNPs by means of cryogenic electron microscopy (cryo-EM). The cryo-EM structure reveals the architecture and organization of the native RNP, defining the attributes of its largely helical structure and how polymerase interacts with nucleoprotein and the viral genome. Observations of branched-RNP structures in negative-stain electron microscopy and their putative identification as replication intermediates suggest a mechanism for viral replication by a second polymerase on the RNP template.

Original languageEnglish
JournalScience
Volume338
Issue6114
Pages (from-to)1631-4
Number of pages4
ISSN0036-8075
DOIs
Publication statusPublished - 21 Dec 2012
Externally publishedYes

    Research areas

  • Cryoelectron Microscopy, Crystallography, X-Ray, Genome, Viral, Image Processing, Computer-Assisted, Influenza A Virus, H1N1 Subtype, Microscopy, Electron, Models, Molecular, Nucleic Acid Conformation, Protein Conformation, Protein Subunits, RNA Replicase, RNA, Viral, RNA-Binding Proteins, Ribonucleoproteins, Transcription, Genetic, Viral Core Proteins, Viral Proteins, Virus Replication

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