Optimizing the precision of laser speckle contrast imaging

Alberto González Olmos, Sharvari Zilpelwar, Smrithi Sunil, David A. Boas, Dmitry D. Postnov*

*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review


Laser speckle contrast imaging (LSCI) is a rapidly developing technology broadly applied for the full-field characterization of tissue perfusion. Over the recent years, significant advancements have been made in interpreting LSCI measurements and improving the technique’s accuracy. On the other hand, the method’s precision has yet to be studied in detail, despite being as important as accuracy for many biomedical applications. Here we combine simulation, theory and animal experiments to systematically evaluate and re-analyze the role of key factors defining LSCI precision—speckle-to-pixel size ratio, polarisation, exposure time and camera-related noise. We show that contrary to the established assumptions, smaller speckle size and shorter exposure time can improve the precision, while the camera choice is less critical and does not affect the signal-to-noise ratio significantly.

Original languageEnglish
Article number17970
JournalScientific Reports
Publication statusPublished - Oct 2023


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