Optimized protocol for isolation of high-quality single cells from the female mouse reproductive tract tissues for single-cell RNA sequencing

Rajendra Kumar Gurumurthy; Naveen Kumar; Cindrilla Chumduri

doi:10.1016/j.xpro.2021.100970

Optimized protocol for isolation of high-quality single cells from the female mouse reproductive tract tissues for single-cell RNA sequencing

Rajendra Kumar Gurumurthy, Naveen Kumar, Cindrilla Chumduri^*

^*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Abstract

Single-cell RNA sequencing (scRNA-seq) is a powerful tool for enumerating the gene expression dynamics at single-cell resolution. Various organs comprising distinct cellular composition and architecture require unique approaches for highly viable single-cell preparation and reliable sequencing results. Here, we describe an optimized protocol for isolating the female reproductive tract (FRT), dissecting different FRT regions, and preparing high-viability single cells from the uterine endocervix and ectocervix to generate a complete molecular cell atlas by scRNA-seq for studying normal physiology and disease. For complete details on the use and execution of this protocol, please refer to Chumduri et al. (2021).

Original language	English
Article number	100970
Journal	STAR Protocols
Volume	2
Issue	4
Number of pages	10
DOIs	https://doi.org/10.1016/j.xpro.2021.100970
Publication status	Published - 17 Dec 2021
Externally published	Yes

Keywords

Cell isolation
RNAseq
Single Cell

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10.1016/j.xpro.2021.100970

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abstract = "Single-cell RNA sequencing (scRNA-seq) is a powerful tool for enumerating the gene expression dynamics at single-cell resolution. Various organs comprising distinct cellular composition and architecture require unique approaches for highly viable single-cell preparation and reliable sequencing results. Here, we describe an optimized protocol for isolating the female reproductive tract (FRT), dissecting different FRT regions, and preparing high-viability single cells from the uterine endocervix and ectocervix to generate a complete molecular cell atlas by scRNA-seq for studying normal physiology and disease. For complete details on the use and execution of this protocol, please refer to Chumduri et al. (2021).",

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Optimized protocol for isolation of high-quality single cells from the female mouse reproductive tract tissues for single-cell RNA sequencing. / Kumar Gurumurthy, Rajendra; Kumar, Naveen; Chumduri, Cindrilla.
In: STAR Protocols, Vol. 2, No. 4, 100970, 17.12.2021.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Optimized protocol for isolation of high-quality single cells from the female mouse reproductive tract tissues for single-cell RNA sequencing

AU - Kumar Gurumurthy, Rajendra

AU - Kumar, Naveen

AU - Chumduri, Cindrilla

PY - 2021/12/17

Y1 - 2021/12/17

N2 - Single-cell RNA sequencing (scRNA-seq) is a powerful tool for enumerating the gene expression dynamics at single-cell resolution. Various organs comprising distinct cellular composition and architecture require unique approaches for highly viable single-cell preparation and reliable sequencing results. Here, we describe an optimized protocol for isolating the female reproductive tract (FRT), dissecting different FRT regions, and preparing high-viability single cells from the uterine endocervix and ectocervix to generate a complete molecular cell atlas by scRNA-seq for studying normal physiology and disease. For complete details on the use and execution of this protocol, please refer to Chumduri et al. (2021).

AB - Single-cell RNA sequencing (scRNA-seq) is a powerful tool for enumerating the gene expression dynamics at single-cell resolution. Various organs comprising distinct cellular composition and architecture require unique approaches for highly viable single-cell preparation and reliable sequencing results. Here, we describe an optimized protocol for isolating the female reproductive tract (FRT), dissecting different FRT regions, and preparing high-viability single cells from the uterine endocervix and ectocervix to generate a complete molecular cell atlas by scRNA-seq for studying normal physiology and disease. For complete details on the use and execution of this protocol, please refer to Chumduri et al. (2021).

KW - Cell isolation

KW - RNAseq

KW - Single Cell

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DO - 10.1016/j.xpro.2021.100970

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JF - STAR Protocols

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M1 - 100970

ER -

Optimized protocol for isolation of high-quality single cells from the female mouse reproductive tract tissues for single-cell RNA sequencing

Abstract

Keywords

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