Optical label-free nanoplasmonic biosensing using a vertical-cavity surface-emitting laser and charge-coupled device

Karin Hedsten, Jordi Fonollosa, Peter Enoksson, Peter Modh, Jörgen Bengtsson, Duncan S Sutherland, Alexandre Dmitriev

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    Abstract

    We present a compact platform for biochemosensing based on the combination of a vertical-cavity surface-emitting laser (VCSEL) light source, microelectromechanical systems (MEMS)-based microoptics, a specially designed nanoplasmonic sensing chip, and charge-coupled device (CCD) detector. The platform does not require any spectral analyzer for signal evaluation, showing good promise for facile integration, neither does it use any microscope setup for the signal collection or imaging. The analytical capabilities of the developed biochemosensing platform are demonstrated by evaluation of the protein-substrate (biotinylated bovine serum albumin-gold) and the protein-protein (biotin-NeutrAvidin) binding kinetics, which is further compared to detection based on conventional optical extinction spectroscopy. The instrument is able to detect low femtomoles of adsorbed proteins with the limit of detection comparable to the state-of-the-art research and commercial optical label-free biochemosensors.
    Original languageEnglish
    JournalAnalytical Chemistry
    Volume82
    Issue4
    Pages (from-to)1535-9
    Number of pages5
    ISSN0003-2700
    DOIs
    Publication statusPublished - 15 Feb 2010

    Keywords

    • Adsorption
    • Animals
    • Avidin
    • Biosensing Techniques
    • Biotin
    • Cattle
    • Gold
    • Kinetics
    • Lasers
    • Light
    • Nanotechnology
    • Optical Processes
    • Serum Albumin, Bovine
    • Staining and Labeling
    • Surface Properties

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