Aarhus University Seal

Optical coherence tomography of arteriolar diameter and capillary perfusion during spreading depolarizations

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Maryam Anzabi, Harvard University
  • ,
  • Baoqiang Li, Chinese Academy of Sciences
  • ,
  • Hui Wang, Harvard University
  • ,
  • Sreekanth Kura, Harvard University
  • ,
  • Sava Sakadžić, Shenzhen Fundamental Research Institutions
  • ,
  • David Boas
  • Leif Østergaard
  • Cenk Ayata, Harvard University, Boston University

Spreading depolarization (SD) is associated with profound oligemia and reduced oxygen availability in the mouse cortex during the depolarization phase. Coincident pial arteriolar constriction has been implicated as the primary mechanism for the oligemia. However, where in the vascular bed the hemodynamic response starts has been unclear. To resolve the origin of the hemodynamic response, we used optical coherence tomography (OCT) to simultaneously monitor changes in the vascular tree from capillary bed to pial arteries in mice during two consecutive SDs 15 minutes apart. We found that capillary flow dropped several seconds before pial arteriolar constriction. Moreover, penetrating arterioles constricted before pial arteries suggesting upstream propagation of constriction. Smaller caliber distal pial arteries constricted stronger than larger caliber proximal arterioles, suggesting that the farther the constriction propagates, the weaker it gets. Altogether, our data indicate that the hemodynamic response to cortical SD originates in the capillary bed.

Original languageEnglish
JournalJournal of Cerebral Blood Flow and Metabolism
Pages (from-to)2256-2263
Number of pages8
Publication statusPublished - Sept 2021

    Research areas

  • Cerebral blood flow, microcirculation, mouse, optical imaging, spreading depolarization, DEPRESSION, VELOCITY, ISCHEMIA, FLUX, HYPOPERFUSION, METABOLISM, CEREBRAL-BLOOD-FLOW, BRAIN, COMMUNICATION, PERICYTES

See relations at Aarhus University Citationformats

ID: 223026441