Opportunities for biomineralization research using multiscale computed X-ray tomography as exemplified by bone imaging

Nina Kølln Wittig; Maja Østergaard; Jonas Palle; Thorbjørn Erik Køppen Christensen; Bente Lomholt Langdahl; Lars Rejnmark; Ellen Margrethe Hauge; Annemarie Brüel; Jesper Skovhus Thomsen; Henrik Birkedal

doi:10.1016/j.jsb.2021.107822

Opportunities for biomineralization research using multiscale computed X-ray tomography as exemplified by bone imaging

Nina Kølln Wittig^*, Maja Østergaard, Jonas Palle, Thorbjørn Erik Køppen Christensen, Bente Lomholt Langdahl, Lars Rejnmark, Ellen Margrethe Hauge, Annemarie Brüel, Jesper Skovhus Thomsen^*, Henrik Birkedal^*

^*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Review › Research › peer-review

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Abstract

Biominerals typically have complex hierarchical structures traversing many length scales. This makes their structural characterization complicated, since it requires 3D techniques that can probe full specimens at down to nanometer-resolution, a combination that is difficult – if not impossible – to achieve simultaneously. One challenging example is bone, a mineralized tissue with a highly complex architecture that is replete with a network of cells. X-ray computed tomography techniques enable multiscale structural characterization through the combination of various equipment and emerge as promising tools for characterizing biominerals. Using bone as an example, we discuss how combining different X-ray imaging instruments allow characterizing bone structures from the nano- to the organ-scale. In particular, we compare and contrast human and rodent bone, emphasize the importance of the osteocyte lacuno-canalicular network in bone, and finally illustrate how combining synchrotron X-ray imaging with laboratory instrumentation for computed tomography is especially helpful for multiscale characterization of biominerals.

Original language	English
Article number	107822
Journal	Journal of Structural Biology
Volume	214
Issue	1
Number of pages	7
ISSN	1047-8477
DOIs	https://doi.org/10.1016/j.jsb.2021.107822
Publication status	Published - Mar 2022

Keywords

Biomineralization
Bone
Bone structure
Computed X-ray tomography
X-ray microscopy
Bone and Bones/diagnostic imaging
Synchrotrons
Osteocytes
Tomography, X-Ray Computed
Imaging, Three-Dimensional

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10.1016/j.jsb.2021.107822Licence: CC BY

1-s2.0-S1047847721001271-mainFinal published version, 8.66 MBLicence: CC BY

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Wittig, N. K., Østergaard, M., Palle, J., Christensen, T. E. K., Langdahl, B. L., Rejnmark, L., Hauge, E. M., Brüel, A., Thomsen, J. S., & Birkedal, H. (2022). Opportunities for biomineralization research using multiscale computed X-ray tomography as exemplified by bone imaging. Journal of Structural Biology, 214(1), Article 107822. https://doi.org/10.1016/j.jsb.2021.107822

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title = "Opportunities for biomineralization research using multiscale computed X-ray tomography as exemplified by bone imaging",

abstract = "Biominerals typically have complex hierarchical structures traversing many length scales. This makes their structural characterization complicated, since it requires 3D techniques that can probe full specimens at down to nanometer-resolution, a combination that is difficult – if not impossible – to achieve simultaneously. One challenging example is bone, a mineralized tissue with a highly complex architecture that is replete with a network of cells. X-ray computed tomography techniques enable multiscale structural characterization through the combination of various equipment and emerge as promising tools for characterizing biominerals. Using bone as an example, we discuss how combining different X-ray imaging instruments allow characterizing bone structures from the nano- to the organ-scale. In particular, we compare and contrast human and rodent bone, emphasize the importance of the osteocyte lacuno-canalicular network in bone, and finally illustrate how combining synchrotron X-ray imaging with laboratory instrumentation for computed tomography is especially helpful for multiscale characterization of biominerals.",

keywords = "Biomineralization, Bone, Bone structure, Computed X-ray tomography, X-ray microscopy, Bone and Bones/diagnostic imaging, Synchrotrons, Osteocytes, Tomography, X-Ray Computed, Imaging, Three-Dimensional",

author = "Wittig, {Nina K{\o}lln} and Maja {\O}stergaard and Jonas Palle and Christensen, {Thorbj{\o}rn Erik K{\o}ppen} and Langdahl, {Bente Lomholt} and Lars Rejnmark and Hauge, {Ellen Margrethe} and Annemarie Br{\"u}el and Thomsen, {Jesper Skovhus} and Henrik Birkedal",

year = "2022",

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doi = "10.1016/j.jsb.2021.107822",

language = "English",

volume = "214",

journal = "Journal of Structural Biology",

issn = "1047-8477",

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Opportunities for biomineralization research using multiscale computed X-ray tomography as exemplified by bone imaging. / Wittig, Nina Kølln; Østergaard, Maja; Palle, Jonas et al.
In: Journal of Structural Biology, Vol. 214, No. 1, 107822, 03.2022.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Review › Research › peer-review

TY - JOUR

T1 - Opportunities for biomineralization research using multiscale computed X-ray tomography as exemplified by bone imaging

AU - Wittig, Nina Kølln

AU - Østergaard, Maja

AU - Palle, Jonas

AU - Christensen, Thorbjørn Erik Køppen

AU - Langdahl, Bente Lomholt

AU - Rejnmark, Lars

AU - Hauge, Ellen Margrethe

AU - Brüel, Annemarie

AU - Thomsen, Jesper Skovhus

AU - Birkedal, Henrik

PY - 2022/3

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N2 - Biominerals typically have complex hierarchical structures traversing many length scales. This makes their structural characterization complicated, since it requires 3D techniques that can probe full specimens at down to nanometer-resolution, a combination that is difficult – if not impossible – to achieve simultaneously. One challenging example is bone, a mineralized tissue with a highly complex architecture that is replete with a network of cells. X-ray computed tomography techniques enable multiscale structural characterization through the combination of various equipment and emerge as promising tools for characterizing biominerals. Using bone as an example, we discuss how combining different X-ray imaging instruments allow characterizing bone structures from the nano- to the organ-scale. In particular, we compare and contrast human and rodent bone, emphasize the importance of the osteocyte lacuno-canalicular network in bone, and finally illustrate how combining synchrotron X-ray imaging with laboratory instrumentation for computed tomography is especially helpful for multiscale characterization of biominerals.

AB - Biominerals typically have complex hierarchical structures traversing many length scales. This makes their structural characterization complicated, since it requires 3D techniques that can probe full specimens at down to nanometer-resolution, a combination that is difficult – if not impossible – to achieve simultaneously. One challenging example is bone, a mineralized tissue with a highly complex architecture that is replete with a network of cells. X-ray computed tomography techniques enable multiscale structural characterization through the combination of various equipment and emerge as promising tools for characterizing biominerals. Using bone as an example, we discuss how combining different X-ray imaging instruments allow characterizing bone structures from the nano- to the organ-scale. In particular, we compare and contrast human and rodent bone, emphasize the importance of the osteocyte lacuno-canalicular network in bone, and finally illustrate how combining synchrotron X-ray imaging with laboratory instrumentation for computed tomography is especially helpful for multiscale characterization of biominerals.

KW - Biomineralization

KW - Bone

KW - Bone structure

KW - Computed X-ray tomography

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KW - Bone and Bones/diagnostic imaging

KW - Synchrotrons

KW - Osteocytes

KW - Tomography, X-Ray Computed

KW - Imaging, Three-Dimensional

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DO - 10.1016/j.jsb.2021.107822

M3 - Review

C2 - 34902560

SN - 1047-8477

VL - 214

JO - Journal of Structural Biology

JF - Journal of Structural Biology

IS - 1

M1 - 107822

ER -

Opportunities for biomineralization research using multiscale computed X-ray tomography as exemplified by bone imaging

Abstract

Keywords

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