Oesteogenesis of Human Induced Pluripotent Stem Cells Derived Mesenchymal Stem Cells on hydroxyapatite contained Nanofibers

TY - JOUR

T1 - Oesteogenesis of Human Induced Pluripotent Stem Cells Derived Mesenchymal Stem Cells on hydroxyapatite contained Nanofibers

AU - Kang, Ran

AU - Luo, Yonglun

AU - Zou, Lijin

AU - Xie, Lin

AU - Lysdahl, Helle

AU - Jiang, Xiumei

AU - Chen, Chunying

AU - Bolund, Lars

AU - Chen, Menglin

AU - Besenbacher, Flemming

AU - Bunger, Cody Eric

PY - 2014

Y1 - 2014

N2 - Biomimetic nanofibrous scaffolds combined with stem cells are promising strategies for bone tissue engineering. In the present study, we have employed nano-hydroxyapatite (nHAp) contained polycaprolactone (PCL) nanofibers as biomimetic nanofibrous scaffold, and mesenchymal stem cells derived from human induced pluripotent stem cells (hiPS-MSCs) as the novel stem cells sources. The response of hiPS-MSCs on the nanofibrous scaffolds in terms of cell proliferation and differentiation into the osteoblastic phenotype was investigated by XTT assay, scanning electron microscopy(SEM), osteogenic genes expression (runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), collagen I (COL1A1), and osteocalcin (OC)), ALP activity, and calcium deposition. It is clearly showed that the hiPS-MSCs attached, and proliferated on the nanofibrous scaffolds. Compared with PCL nanofibers without nHAp, the cells on the nHAp contained nanofibers demonstrated superior capabilites to differentiate to form calcified extracellular matrix. Together with gene expression, all the results indicate the great potential of the hiPS-MSCs seeded biomimetic nanofibrous scaffolds for bone regeneration in the future.

AB - Biomimetic nanofibrous scaffolds combined with stem cells are promising strategies for bone tissue engineering. In the present study, we have employed nano-hydroxyapatite (nHAp) contained polycaprolactone (PCL) nanofibers as biomimetic nanofibrous scaffold, and mesenchymal stem cells derived from human induced pluripotent stem cells (hiPS-MSCs) as the novel stem cells sources. The response of hiPS-MSCs on the nanofibrous scaffolds in terms of cell proliferation and differentiation into the osteoblastic phenotype was investigated by XTT assay, scanning electron microscopy(SEM), osteogenic genes expression (runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), collagen I (COL1A1), and osteocalcin (OC)), ALP activity, and calcium deposition. It is clearly showed that the hiPS-MSCs attached, and proliferated on the nanofibrous scaffolds. Compared with PCL nanofibers without nHAp, the cells on the nHAp contained nanofibers demonstrated superior capabilites to differentiate to form calcified extracellular matrix. Together with gene expression, all the results indicate the great potential of the hiPS-MSCs seeded biomimetic nanofibrous scaffolds for bone regeneration in the future.

U2 - 10.1039/C3RA44181D

DO - 10.1039/C3RA44181D

M3 - Journal article

SN - 2046-2069

VL - 4

SP - 5734

EP - 5739

JO - R S C Advances

JF - R S C Advances

IS - 11

ER -