Novel fluorescent nanoparticles for ultrasensitive identification of nucleic acids by optical methods

Research output: Contribution to book/anthology/report/proceedingArticle in proceedingsResearchpeer-review

  • Mads Westergaard Mulberg, Syddansk universitet
  • ,
  • Maria Taskova, Syddansk universitet
  • ,
  • Rasmus P. Thomsen
  • ,
  • Anders H. Okholm
  • ,
  • Jørgen Kjems
  • Kira Astakhova, Syddansk universitet

For decades, the detection of nucleic acids and their interactions at low abundances has been a challenging task. Present nucleic acid diagnostics are primarily based on enzymatic reactions including sequencing, polymerase-chain reaction and microarrays. However, the use of enzymatic amplification interferes with the initial biomolecular system, is limited to in vitro assays, often time consuming and rather expensive. Therefore, there is interest in new amplification-free detection methods. A tremendous progress has been made in fluorescence based optical detection of biomolecules. In this work, we aimed at developing efficient tools for amplification-free nucleic acid detection. The result of simple and inexpensive polymerization in the presence of fluorescent dyes and additional functionalization reagents was ultra-bright fluorescent nanoparticles modified with additional groups for bioconjugation.

Original languageEnglish
Title of host publicationBiotech, Biomaterials and Biomedical - TechConnect Briefs 2017
Number of pages4
Volume3
PublisherTechConnect
Publication year1 Jan 2017
Pages118-121
ISBN (Electronic)9780998878201
Publication statusPublished - 1 Jan 2017
Event11th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 20th Annual Nanotech Conference and Expo, and the 2017 National SBIR/STTR Conference - Washington, United States
Duration: 14 May 201717 May 2017

Conference

Conference11th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 20th Annual Nanotech Conference and Expo, and the 2017 National SBIR/STTR Conference
LandUnited States
ByWashington
Periode14/05/201717/05/2017

    Research areas

  • Butyl acrylate, Fluorescence, Nanoparticle, Nucleic acid, Radical polymerization

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