Novel fluorescent nanoparticles for ultrasensitive identification of nucleic acids by optical methods

TY - GEN

T1 - Novel fluorescent nanoparticles for ultrasensitive identification of nucleic acids by optical methods

AU - Mulberg, Mads Westergaard

AU - Taskova, Maria

AU - Thomsen, Rasmus P.

AU - Okholm, Anders H.

AU - Kjems, Jørgen

AU - Astakhova, Kira

PY - 2017/1/1

Y1 - 2017/1/1

N2 - For decades, the detection of nucleic acids and their interactions at low abundances has been a challenging task. Present nucleic acid diagnostics are primarily based on enzymatic reactions including sequencing, polymerase-chain reaction and microarrays. However, the use of enzymatic amplification interferes with the initial biomolecular system, is limited to in vitro assays, often time consuming and rather expensive. Therefore, there is interest in new amplification-free detection methods. A tremendous progress has been made in fluorescence based optical detection of biomolecules. In this work, we aimed at developing efficient tools for amplification-free nucleic acid detection. The result of simple and inexpensive polymerization in the presence of fluorescent dyes and additional functionalization reagents was ultra-bright fluorescent nanoparticles modified with additional groups for bioconjugation.

AB - For decades, the detection of nucleic acids and their interactions at low abundances has been a challenging task. Present nucleic acid diagnostics are primarily based on enzymatic reactions including sequencing, polymerase-chain reaction and microarrays. However, the use of enzymatic amplification interferes with the initial biomolecular system, is limited to in vitro assays, often time consuming and rather expensive. Therefore, there is interest in new amplification-free detection methods. A tremendous progress has been made in fluorescence based optical detection of biomolecules. In this work, we aimed at developing efficient tools for amplification-free nucleic acid detection. The result of simple and inexpensive polymerization in the presence of fluorescent dyes and additional functionalization reagents was ultra-bright fluorescent nanoparticles modified with additional groups for bioconjugation.

KW - Butyl acrylate

KW - Fluorescence

KW - Nanoparticle

KW - Nucleic acid

KW - Radical polymerization

UR - http://www.scopus.com/inward/record.url?scp=85029350411&partnerID=8YFLogxK

M3 - Article in proceedings

AN - SCOPUS:85029350411

VL - 3

SP - 118

EP - 121

BT - Biotech, Biomaterials and Biomedical - TechConnect Briefs 2017

PB - TechConnect

T2 - 11th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 20th Annual Nanotech Conference and Expo, and the 2017 National SBIR/STTR Conference

Y2 - 14 May 2017 through 17 May 2017

ER -