Non-ablative doses of focal ionizing radiation alters function of central neural circuits

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Hamed Zaer
  • Wei Fan, Leibniz Institute for Resilience Research
  • ,
  • Dariusz Orlowski
  • Andreas N. Glud
  • Morten B. Jensen
  • Esben S. Worm
  • ,
  • Slávka Lukacova
  • Trine W. Mikkelsen
  • Lise M. Fitting, Aarhus University
  • ,
  • Liisa M. Jacobsen
  • Thomas Portmann, Lineagen Inc
  • ,
  • Jui Yi Hsieh, Lineagen Inc
  • ,
  • Christopher Noel, Lineagen Inc
  • ,
  • Georg Weidlich, Lineagen Inc
  • ,
  • Woody Chung, Lineagen Inc
  • ,
  • Patrick Riley, Lineagen Inc
  • ,
  • Cesare Jenkins, Lineagen Inc
  • ,
  • John R. Adler, Zap Surgical Systems, Inc., Lineagen Inc, Stanford University
  • ,
  • M. Bret Schneider, Zap Surgical Systems, Inc., Lineagen Inc, Stanford University
  • ,
  • Jens Christian H. Sørensen
  • Albrecht Stroh, Leibniz Institute for Resilience Research, Johannes Gutenberg University Mainz

Background: Modulation of pathological neural circuit activity in the brain with a minimum of complications is an area of intense interest. Objective: The goal of the study was to alter neurons' physiological states without apparent damage of cellular integrity using stereotactic radiosurgery (SRS). Methods: We treated a 7.5 mm-diameter target on the visual cortex of Göttingen minipigs with doses of 40, 60, 80, and 100 Gy. Six months post-irradiation, the pigs were implanted with a 9 mm-wide, eight-shank multi-electrode probe, which spanned the radiation focus as well as the low-exposure neighboring areas. Results: Doses of 40 Gy led to an increase of spontaneous firing rate, six months post-irradiation, while doses of 60 Gy and greater were associated with a decrease. Subjecting the animals to visual stimuli resulted in typical visual evoked potentials (VEP). At 40 Gy, a significant reduction of the P1 peak time, indicative of higher network excitability was observed. At 80 Gy, P1 peak time was not affected, while a minor reduction at 60 Gy was seen. No distance-dependent effects on spontaneous firing rate, or on VEP were observed. Post-mortem histology revealed no evidence of necrosis at doses below 60 Gy. In an in vitro assay comprising of iPS-derived human neuron-astrocyte co-cultures, we found a higher vulnerability of inhibitory neurons than excitatory neurons with respect to radiation, which might provide the cellular mechanism of the disinhibitory effect observed in vivo. Conclusion: We provide initial evidence for a rather circuit-wide, long-lasting disinhibitory effect of low sub-ablative doses of SRS.

Original languageEnglish
JournalBrain Stimulation
Volume15
Issue3
Pages (from-to)586-597
Number of pages12
ISSN1935-861X
DOIs
Publication statusPublished - May 2022

    Research areas

  • Minipig, Neuromodulation, Spontaneous firing rate, Stereotactic radiosurgery, Visual cortex, Visual evoked potential

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