No Differences in Cerebral Immunohistochemical Markers following Remote Ischemic Postconditioning in Newborn Piglets with Hypoxia-Ischemia

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No Differences in Cerebral Immunohistochemical Markers following Remote Ischemic Postconditioning in Newborn Piglets with Hypoxia-Ischemia. / Brogård Andersen, Hannah; Andersen, Mads; Bennedsgaard, Kristine Tang et al.

In: Neuropediatrics, Vol. 53, No. 6, 2022, p. 423-431.

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@article{2012f7bd38924e1e9b2bdcd42acce038,
title = "No Differences in Cerebral Immunohistochemical Markers following Remote Ischemic Postconditioning in Newborn Piglets with Hypoxia-Ischemia",
abstract = "Background  Despite therapeutic hypothermia, neonates with hypoxic-ischemic encephalopathy still develop neurological disabilities. We have previously investigated neuroprotection by remote ischemic postconditioning (RIPC) in newborn piglets following hypoxia-ischemia (HI). The aim of this study was to further investigate potential effects of RIPC on cerebral immunohistochemical markers related to edema, apoptosis, and angiogenesis. Methods  Brain expression of aquaporin 4, caspase-3, B-cell lymphoma 2, and vascular endothelial growth factor was analyzed by immunohistochemistry in 23 piglets, randomly selected from a larger study of RIPC after HI. Twenty animals were subjected to 45 minutes of HI and randomized to treatment with and without RIPC, while three animals were randomized to sham procedures. RIPC was conducted by four conditioning cycles of 5-minute ischemia and reperfusion. Piglets were euthanized 72 hours after the HI insult. Results  Piglets subjected to HI treated with and without RIPC were similar at baseline and following the HI insult. However, piglets randomized to HI alone had longer duration of low blood pressure during the insult. We found no differences in the brain expression of the immunohistochemical markers in any regions of interest or the whole brain between the two HI groups. Conclusion  RIPC did not influence brain expression of markers related to edema, apoptosis, or angiogenesis in newborn piglets at 72 hours after HI. These results support previous findings of limited neuroprotective effect by this RIPC protocol. Our results may have been affected by the time of assessment, use of fentanyl as anesthetic, or limitations related to our immunohistochemical methods.",
keywords = "Hypoxia-ischemia, hypoxic-ischemic encephalopathy, immunohistochemistry, piglet model, remote ischemic postconditioning, Animals, Newborn, Vascular Endothelial Growth Factor A, Hypoxia-Ischemia, Brain/pathology, Humans, Animals, Ischemia, Swine, Biomarkers, Hypoxia, Ischemic Postconditioning/methods, Disease Models, Animal",
author = "{Brog{\aa}rd Andersen}, Hannah and Mads Andersen and Bennedsgaard, {Kristine Tang} and Sigrid Kerrn-Jespersen and {Jacobsen Kyng}, Kasper and Holm, {Ida Elisabeth} and Henriksen, {Tine Brink}",
year = "2022",
doi = "10.1055/a-1889-8544",
language = "English",
volume = "53",
pages = "423--431",
journal = "Neuropediatrics",
issn = "0174-304X",
publisher = "GeorgThieme Verlag",
number = "6",

}

RIS

TY - JOUR

T1 - No Differences in Cerebral Immunohistochemical Markers following Remote Ischemic Postconditioning in Newborn Piglets with Hypoxia-Ischemia

AU - Brogård Andersen, Hannah

AU - Andersen, Mads

AU - Bennedsgaard, Kristine Tang

AU - Kerrn-Jespersen, Sigrid

AU - Jacobsen Kyng, Kasper

AU - Holm, Ida Elisabeth

AU - Henriksen, Tine Brink

PY - 2022

Y1 - 2022

N2 - Background  Despite therapeutic hypothermia, neonates with hypoxic-ischemic encephalopathy still develop neurological disabilities. We have previously investigated neuroprotection by remote ischemic postconditioning (RIPC) in newborn piglets following hypoxia-ischemia (HI). The aim of this study was to further investigate potential effects of RIPC on cerebral immunohistochemical markers related to edema, apoptosis, and angiogenesis. Methods  Brain expression of aquaporin 4, caspase-3, B-cell lymphoma 2, and vascular endothelial growth factor was analyzed by immunohistochemistry in 23 piglets, randomly selected from a larger study of RIPC after HI. Twenty animals were subjected to 45 minutes of HI and randomized to treatment with and without RIPC, while three animals were randomized to sham procedures. RIPC was conducted by four conditioning cycles of 5-minute ischemia and reperfusion. Piglets were euthanized 72 hours after the HI insult. Results  Piglets subjected to HI treated with and without RIPC were similar at baseline and following the HI insult. However, piglets randomized to HI alone had longer duration of low blood pressure during the insult. We found no differences in the brain expression of the immunohistochemical markers in any regions of interest or the whole brain between the two HI groups. Conclusion  RIPC did not influence brain expression of markers related to edema, apoptosis, or angiogenesis in newborn piglets at 72 hours after HI. These results support previous findings of limited neuroprotective effect by this RIPC protocol. Our results may have been affected by the time of assessment, use of fentanyl as anesthetic, or limitations related to our immunohistochemical methods.

AB - Background  Despite therapeutic hypothermia, neonates with hypoxic-ischemic encephalopathy still develop neurological disabilities. We have previously investigated neuroprotection by remote ischemic postconditioning (RIPC) in newborn piglets following hypoxia-ischemia (HI). The aim of this study was to further investigate potential effects of RIPC on cerebral immunohistochemical markers related to edema, apoptosis, and angiogenesis. Methods  Brain expression of aquaporin 4, caspase-3, B-cell lymphoma 2, and vascular endothelial growth factor was analyzed by immunohistochemistry in 23 piglets, randomly selected from a larger study of RIPC after HI. Twenty animals were subjected to 45 minutes of HI and randomized to treatment with and without RIPC, while three animals were randomized to sham procedures. RIPC was conducted by four conditioning cycles of 5-minute ischemia and reperfusion. Piglets were euthanized 72 hours after the HI insult. Results  Piglets subjected to HI treated with and without RIPC were similar at baseline and following the HI insult. However, piglets randomized to HI alone had longer duration of low blood pressure during the insult. We found no differences in the brain expression of the immunohistochemical markers in any regions of interest or the whole brain between the two HI groups. Conclusion  RIPC did not influence brain expression of markers related to edema, apoptosis, or angiogenesis in newborn piglets at 72 hours after HI. These results support previous findings of limited neuroprotective effect by this RIPC protocol. Our results may have been affected by the time of assessment, use of fentanyl as anesthetic, or limitations related to our immunohistochemical methods.

KW - Hypoxia-ischemia

KW - hypoxic-ischemic encephalopathy

KW - immunohistochemistry

KW - piglet model

KW - remote ischemic postconditioning

KW - Animals, Newborn

KW - Vascular Endothelial Growth Factor A

KW - Hypoxia-Ischemia, Brain/pathology

KW - Humans

KW - Animals

KW - Ischemia

KW - Swine

KW - Biomarkers

KW - Hypoxia

KW - Ischemic Postconditioning/methods

KW - Disease Models, Animal

U2 - 10.1055/a-1889-8544

DO - 10.1055/a-1889-8544

M3 - Journal article

C2 - 35777661

VL - 53

SP - 423

EP - 431

JO - Neuropediatrics

JF - Neuropediatrics

SN - 0174-304X

IS - 6

ER -