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NHE-1 sequence and expression in toad, snake and fish red blood cells

Research output: Contribution to conferencePosterResearch

  • Steffen Nyegaard Thomsen, Denmark
  • Tobias Wang
  • Torsten Kristensen, Denmark
  • Department of Molecular Biology
  • Department of Biological Sciences, Zoophysiology
Red blood cells (RBC) from reptiles appear not to express regulatory volume increase (RVI) upon shrinkage (Kristensen et al., 2008). In other vertebrates, the RVI response is primarily mediated by activation of the Na+/H+ exchanger (NHE-1) and we, therefore decided to investigate whether red cells of reptiles express a different NHE-1 that responds less to volume activation compared to other vertebrates or simply lack the Na+/H+ exchanger. Using various tissues from the ball python (Python regius), Cane toad (Bufo marinus) and European perch (Perca fluviatilis), cDNA libraries were created and sequenced using PCR with primers generated from a clustal alignment of available NHE-1 sequences in the NR database. Having established the presence of the NHE-1 gene in python, cane toad and perch, expression was quantified by Western Blot and Immunoconfocal microscopy using fluorophore coupled NHE-1 antibodies. These studies showed that NHE-1 is expressed in RBCs from both toads and teleosts fish, but is not expressed in the RBCs of pythons. Thus, the lack of the RVI response in pythons is likely to reflect the lack of capacity to exchange Na+ and H+ for volume regulation.
Original languageEnglish
Publication year2010
Number of pages1
Publication statusPublished - 2010
Event42nd Sandbjerg meeting on membrane transport - Sønderborg, Denmark
Duration: 19 May 201021 May 2010

Conference

Conference42nd Sandbjerg meeting on membrane transport
CountryDenmark
CitySønderborg
Period19/05/201021/05/2010

    Research areas

  • NHE-1, SLC9A1, Na-H transport, Volume regulation, pH regulation

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