Neurotoxicity of pre-incubated alpha-synuclein with neutral nanoliposomes on PC12 and SHSY5Y cell lines

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

DOI

  • F. Aliakbari, Semnan University of Medical Sciences
  • ,
  • A. A. Shabani, Semnan University of Medical Sciences
  • ,
  • H. Bardania, Yasuj University of Medical Sciences
  • ,
  • H. A.Eslampanah Seyedi, National Institute of Genetic Engineering and Biotechnology
  • ,
  • H. Mohammad-Beigie
  • A. Tayaranian Marvian, National Institute of Genetic Engineering and Biotechnology
  • ,
  • M. Nasouti, National Institute of Genetic Engineering and Biotechnology
  • ,
  • A. A. Vafaei, Semnan University of Medical Sciences
  • ,
  • S. A. Shojaosadati, Tarbiat Modares University
  • ,
  • A. A. Saboury, Tehran University
  • ,
  • G. Christiansen
  • D. Morshedi, National Institute of Genetic Engineering and Biotechnology

Alpha-synuclein (α-Syn) is a protein with high tendency to convert into toxic aggregates that are involved in Parkinson's Disease (PD). The conversion of α-Syn into toxic aggregates may associate with bio-membranes and the interaction between α-Syn and bio-mimic liposomes can trigger the neurotoxicity. Some inhibitors of α-Syn aggregation are challengeable drugs. Using nano carriers such as nanoliposomes may overcome such challenges. Nevertheless, there are few studies on nanoliposomes effects on neuronal cells and α-Syn. Herein, we investigated the neurotoxicity and fibrinogenesis of neutral charged nanoliposomes. Thin layer evaporation method and hydration method was employed to formulate the nanoliposomes from Dipalmitoylphosphatidylcholine to the size of ≤ 100 nm. Fibrillation and cytotoxicity of α-Syn treated with nanoliposome were measured. Neither neurotoxicity nor fibrillation was observed when α-Syn treated with different concentrations of nanoliposome. These results well indicate that nanoliposome at the concentrations required for drug delivery not only is able to prevent fibrillation process, but also has no considerable toxic effects on the cells.

Original languageEnglish
JournalScientia Iranica: international journal of science & technology
Volume24
Issue6
Pages (from-to)3542-3553
Number of pages12
ISSN1026-3098
DOIs
Publication statusPublished - 2017

    Research areas

  • Alpha-synuclein, Amyloid-related diseases, Fibrillation, Nanoliposome, Neurotoxicity, Parkinson's disease

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