Neurodevelopmental defects in a mouse model of O-GlcNAc transferase intellectual disability

Florence Authier, Nina Ondruskova, Andrew T. Ferenbach, Alison D. McNeilly, Daan M.F. van Aalten

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Abstract

The addition of O-linked β-N-acetylglucosamine (O-GlcNAc) to proteins (referred to as O-GlcNAcylation) is a modification that is crucial for vertebrate development. O-GlcNAcylation is catalyzed by O-GlcNAc transferase (OGT) and reversed by O-GlcNAcase (OGA). Missense variants of OGT have recently been shown to segregate with an X-linked syndromic form of intellectual disability, OGT-linked congenital disorder of glycosylation (OGT-CDG). Although the existence of OGT-CDG suggests that O-GlcNAcylation is crucial for neurodevelopment and/or cognitive function, the underlying pathophysiologic mechanisms remain unknown. Here we report a mouse line that carries a catalytically impaired OGT-CDG variant. These mice show altered O-GlcNAc homeostasis with decreased global O-GlcNAcylation and reduced levels of OGT and OGA in the brain. Phenotypic characterization of the mice revealed lower body weight associated with reduced body fat mass, short stature and microcephaly. This mouse model will serve as an important tool to study genotype-phenotype correlations in OGT-CDG in vivo and for the development of possible treatment avenues for this disorder.

Original languageEnglish
Article numberdmm050671
JournalDisease Models & Mechanisms
Volume17
Issue4
ISSN1754-8403
DOIs
Publication statusPublished - 1 Apr 2024

Keywords

  • O-GlcNAcylation
  • Intellectual disability
  • Vertebrate development
  • Body Weight
  • N-Acetylglucosaminyltransferases/metabolism
  • Glycosylation
  • Neurodevelopmental Disorders/pathology
  • Intellectual Disability/genetics
  • Brain/pathology
  • Phenotype
  • Animals
  • Mice
  • beta-N-Acetylhexosaminidases/metabolism
  • Disease Models, Animal

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