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Negative regulation of CCaMK is essential for symbiotic infection

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Negative regulation of CCaMK is essential for symbiotic infection. / Liao, Jinqiu; Singh, Sylvia; Hossain, Md S; Andersen, Stig Uggerhøj; Ross, Loretta; Bonetta, Dario; Zhou, Yonghong; Sato, Shusei; Tabata, Satoshi; Stougaard, Jens; Szczyglowski, Krzysztof; Parniske, Martin.

In: Plant Journal, Vol. 72, No. 4, 11.2012, p. 572-584.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Liao, J, Singh, S, Hossain, MS, Andersen, SU, Ross, L, Bonetta, D, Zhou, Y, Sato, S, Tabata, S, Stougaard, J, Szczyglowski, K & Parniske, M 2012, 'Negative regulation of CCaMK is essential for symbiotic infection', Plant Journal, vol. 72, no. 4, pp. 572-584. https://doi.org/10.1111/j.1365-313X.2012.05098.x

APA

Liao, J., Singh, S., Hossain, M. S., Andersen, S. U., Ross, L., Bonetta, D., ... Parniske, M. (2012). Negative regulation of CCaMK is essential for symbiotic infection. Plant Journal, 72(4), 572-584. https://doi.org/10.1111/j.1365-313X.2012.05098.x

CBE

Liao J, Singh S, Hossain MS, Andersen SU, Ross L, Bonetta D, Zhou Y, Sato S, Tabata S, Stougaard J, Szczyglowski K, Parniske M. 2012. Negative regulation of CCaMK is essential for symbiotic infection. Plant Journal. 72(4):572-584. https://doi.org/10.1111/j.1365-313X.2012.05098.x

MLA

Vancouver

Liao J, Singh S, Hossain MS, Andersen SU, Ross L, Bonetta D et al. Negative regulation of CCaMK is essential for symbiotic infection. Plant Journal. 2012 Nov;72(4):572-584. https://doi.org/10.1111/j.1365-313X.2012.05098.x

Author

Liao, Jinqiu ; Singh, Sylvia ; Hossain, Md S ; Andersen, Stig Uggerhøj ; Ross, Loretta ; Bonetta, Dario ; Zhou, Yonghong ; Sato, Shusei ; Tabata, Satoshi ; Stougaard, Jens ; Szczyglowski, Krzysztof ; Parniske, Martin. / Negative regulation of CCaMK is essential for symbiotic infection. In: Plant Journal. 2012 ; Vol. 72, No. 4. pp. 572-584.

Bibtex

@article{35a58779b19d4261ac029d87d0a60d5f,
title = "Negative regulation of CCaMK is essential for symbiotic infection",
abstract = "One of the earliest responses of legumes to symbiotic signalling is oscillation of the calcium concentration in the nucleoplasm of root epidermal cells. Integration and decoding of the calcium-spiking signal involve a calcium- and calmodulin-dependent protein kinase (CCaMK) and its phosphorylation substrates, such as CYCLOPS. Here we describe the Lotus japonicus ccamk-14 mutant that originated from a har1-1 suppressor screen. The ccamk-14 mutation causes a serine to asparagine substitution at position 337 located within the calmodulin binding site, which we determined to be an in vitro phosphorylation site in CCaMK. We show that ccamk-14 exerts cell-specific effects on symbiosis. The mutant is characterized by an increased frequency of epidermal infections and significantly compromised cortical infections by Mesorhizobium loti and also the arbuscular mycorrhiza fungus Rhizophagus irregularis. The S337 residue is conserved across angiosperm CCaMKs, and testing discrete substitutions at this site showed that it participates in a negative regulation of CCaMK activity, which is required for the cell-type-specific integration of symbiotic signalling.",
author = "Jinqiu Liao and Sylvia Singh and Hossain, {Md S} and Andersen, {Stig Uggerh{\o}j} and Loretta Ross and Dario Bonetta and Yonghong Zhou and Shusei Sato and Satoshi Tabata and Jens Stougaard and Krzysztof Szczyglowski and Martin Parniske",
note = "{\circledC} 2012 The Authors. The Plant Journal {\circledC} 2012 Blackwell Publishing Ltd.",
year = "2012",
month = "11",
doi = "10.1111/j.1365-313X.2012.05098.x",
language = "English",
volume = "72",
pages = "572--584",
journal = "The Plant Journal",
issn = "0960-7412",
publisher = "Wiley-Blackwell Publishing Ltd.",
number = "4",

}

RIS

TY - JOUR

T1 - Negative regulation of CCaMK is essential for symbiotic infection

AU - Liao, Jinqiu

AU - Singh, Sylvia

AU - Hossain, Md S

AU - Andersen, Stig Uggerhøj

AU - Ross, Loretta

AU - Bonetta, Dario

AU - Zhou, Yonghong

AU - Sato, Shusei

AU - Tabata, Satoshi

AU - Stougaard, Jens

AU - Szczyglowski, Krzysztof

AU - Parniske, Martin

N1 - © 2012 The Authors. The Plant Journal © 2012 Blackwell Publishing Ltd.

PY - 2012/11

Y1 - 2012/11

N2 - One of the earliest responses of legumes to symbiotic signalling is oscillation of the calcium concentration in the nucleoplasm of root epidermal cells. Integration and decoding of the calcium-spiking signal involve a calcium- and calmodulin-dependent protein kinase (CCaMK) and its phosphorylation substrates, such as CYCLOPS. Here we describe the Lotus japonicus ccamk-14 mutant that originated from a har1-1 suppressor screen. The ccamk-14 mutation causes a serine to asparagine substitution at position 337 located within the calmodulin binding site, which we determined to be an in vitro phosphorylation site in CCaMK. We show that ccamk-14 exerts cell-specific effects on symbiosis. The mutant is characterized by an increased frequency of epidermal infections and significantly compromised cortical infections by Mesorhizobium loti and also the arbuscular mycorrhiza fungus Rhizophagus irregularis. The S337 residue is conserved across angiosperm CCaMKs, and testing discrete substitutions at this site showed that it participates in a negative regulation of CCaMK activity, which is required for the cell-type-specific integration of symbiotic signalling.

AB - One of the earliest responses of legumes to symbiotic signalling is oscillation of the calcium concentration in the nucleoplasm of root epidermal cells. Integration and decoding of the calcium-spiking signal involve a calcium- and calmodulin-dependent protein kinase (CCaMK) and its phosphorylation substrates, such as CYCLOPS. Here we describe the Lotus japonicus ccamk-14 mutant that originated from a har1-1 suppressor screen. The ccamk-14 mutation causes a serine to asparagine substitution at position 337 located within the calmodulin binding site, which we determined to be an in vitro phosphorylation site in CCaMK. We show that ccamk-14 exerts cell-specific effects on symbiosis. The mutant is characterized by an increased frequency of epidermal infections and significantly compromised cortical infections by Mesorhizobium loti and also the arbuscular mycorrhiza fungus Rhizophagus irregularis. The S337 residue is conserved across angiosperm CCaMKs, and testing discrete substitutions at this site showed that it participates in a negative regulation of CCaMK activity, which is required for the cell-type-specific integration of symbiotic signalling.

U2 - 10.1111/j.1365-313X.2012.05098.x

DO - 10.1111/j.1365-313X.2012.05098.x

M3 - Journal article

C2 - 22775286

VL - 72

SP - 572

EP - 584

JO - The Plant Journal

JF - The Plant Journal

SN - 0960-7412

IS - 4

ER -