Negative regulation of CCaMK is essential for symbiotic infection

Jinqiu Liao; Sylvia Singh; Md S Hossain; Stig Uggerhøj Andersen; Loretta Ross; Dario Bonetta; Yonghong Zhou; Shusei Sato; Satoshi Tabata; Jens Stougaard; Krzysztof Szczyglowski; Martin Parniske

doi:10.1111/j.1365-313X.2012.05098.x

Negative regulation of CCaMK is essential for symbiotic infection

Jinqiu Liao, Sylvia Singh, Md S Hossain, Stig Uggerhøj Andersen, Loretta Ross, Dario Bonetta, Yonghong Zhou, Shusei Sato, Satoshi Tabata, Jens Stougaard, Krzysztof Szczyglowski, Martin Parniske

Department of Molecular Biology and Genetics

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Abstract

One of the earliest responses of legumes to symbiotic signalling is oscillation of the calcium concentration in the nucleoplasm of root epidermal cells. Integration and decoding of the calcium-spiking signal involve a calcium- and calmodulin-dependent protein kinase (CCaMK) and its phosphorylation substrates, such as CYCLOPS. Here we describe the Lotus japonicus ccamk-14 mutant that originated from a har1-1 suppressor screen. The ccamk-14 mutation causes a serine to asparagine substitution at position 337 located within the calmodulin binding site, which we determined to be an in vitro phosphorylation site in CCaMK. We show that ccamk-14 exerts cell-specific effects on symbiosis. The mutant is characterized by an increased frequency of epidermal infections and significantly compromised cortical infections by Mesorhizobium loti and also the arbuscular mycorrhiza fungus Rhizophagus irregularis. The S337 residue is conserved across angiosperm CCaMKs, and testing discrete substitutions at this site showed that it participates in a negative regulation of CCaMK activity, which is required for the cell-type-specific integration of symbiotic signalling.

Original language	English
Journal	Plant Journal
Volume	72
Issue	4
Pages (from-to)	572-584
Number of pages	12
ISSN	0960-7412
DOIs	https://doi.org/10.1111/j.1365-313X.2012.05098.x
Publication status	Published - Nov 2012

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@article{35a58779b19d4261ac029d87d0a60d5f,

title = "Negative regulation of CCaMK is essential for symbiotic infection",

abstract = "One of the earliest responses of legumes to symbiotic signalling is oscillation of the calcium concentration in the nucleoplasm of root epidermal cells. Integration and decoding of the calcium-spiking signal involve a calcium- and calmodulin-dependent protein kinase (CCaMK) and its phosphorylation substrates, such as CYCLOPS. Here we describe the Lotus japonicus ccamk-14 mutant that originated from a har1-1 suppressor screen. The ccamk-14 mutation causes a serine to asparagine substitution at position 337 located within the calmodulin binding site, which we determined to be an in vitro phosphorylation site in CCaMK. We show that ccamk-14 exerts cell-specific effects on symbiosis. The mutant is characterized by an increased frequency of epidermal infections and significantly compromised cortical infections by Mesorhizobium loti and also the arbuscular mycorrhiza fungus Rhizophagus irregularis. The S337 residue is conserved across angiosperm CCaMKs, and testing discrete substitutions at this site showed that it participates in a negative regulation of CCaMK activity, which is required for the cell-type-specific integration of symbiotic signalling.",

author = "Jinqiu Liao and Sylvia Singh and Hossain, {Md S} and Andersen, {Stig Uggerh{\o}j} and Loretta Ross and Dario Bonetta and Yonghong Zhou and Shusei Sato and Satoshi Tabata and Jens Stougaard and Krzysztof Szczyglowski and Martin Parniske",

note = "{\textcopyright} 2012 The Authors. The Plant Journal {\textcopyright} 2012 Blackwell Publishing Ltd.",

year = "2012",

month = nov,

doi = "10.1111/j.1365-313X.2012.05098.x",

language = "English",

volume = "72",

pages = "572--584",

journal = "Plant Journal",

issn = "0960-7412",

publisher = "Wiley-Blackwell Publishing Ltd.",

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TY - JOUR

T1 - Negative regulation of CCaMK is essential for symbiotic infection

AU - Liao, Jinqiu

AU - Singh, Sylvia

AU - Hossain, Md S

AU - Andersen, Stig Uggerhøj

AU - Ross, Loretta

AU - Bonetta, Dario

AU - Zhou, Yonghong

AU - Sato, Shusei

AU - Tabata, Satoshi

AU - Stougaard, Jens

AU - Szczyglowski, Krzysztof

AU - Parniske, Martin

PY - 2012/11

Y1 - 2012/11

N2 - One of the earliest responses of legumes to symbiotic signalling is oscillation of the calcium concentration in the nucleoplasm of root epidermal cells. Integration and decoding of the calcium-spiking signal involve a calcium- and calmodulin-dependent protein kinase (CCaMK) and its phosphorylation substrates, such as CYCLOPS. Here we describe the Lotus japonicus ccamk-14 mutant that originated from a har1-1 suppressor screen. The ccamk-14 mutation causes a serine to asparagine substitution at position 337 located within the calmodulin binding site, which we determined to be an in vitro phosphorylation site in CCaMK. We show that ccamk-14 exerts cell-specific effects on symbiosis. The mutant is characterized by an increased frequency of epidermal infections and significantly compromised cortical infections by Mesorhizobium loti and also the arbuscular mycorrhiza fungus Rhizophagus irregularis. The S337 residue is conserved across angiosperm CCaMKs, and testing discrete substitutions at this site showed that it participates in a negative regulation of CCaMK activity, which is required for the cell-type-specific integration of symbiotic signalling.

AB - One of the earliest responses of legumes to symbiotic signalling is oscillation of the calcium concentration in the nucleoplasm of root epidermal cells. Integration and decoding of the calcium-spiking signal involve a calcium- and calmodulin-dependent protein kinase (CCaMK) and its phosphorylation substrates, such as CYCLOPS. Here we describe the Lotus japonicus ccamk-14 mutant that originated from a har1-1 suppressor screen. The ccamk-14 mutation causes a serine to asparagine substitution at position 337 located within the calmodulin binding site, which we determined to be an in vitro phosphorylation site in CCaMK. We show that ccamk-14 exerts cell-specific effects on symbiosis. The mutant is characterized by an increased frequency of epidermal infections and significantly compromised cortical infections by Mesorhizobium loti and also the arbuscular mycorrhiza fungus Rhizophagus irregularis. The S337 residue is conserved across angiosperm CCaMKs, and testing discrete substitutions at this site showed that it participates in a negative regulation of CCaMK activity, which is required for the cell-type-specific integration of symbiotic signalling.

U2 - 10.1111/j.1365-313X.2012.05098.x

DO - 10.1111/j.1365-313X.2012.05098.x

M3 - Journal article

C2 - 22775286

SN - 0960-7412

VL - 72

SP - 572

EP - 584

JO - Plant Journal

JF - Plant Journal

IS - 4

ER -

Negative regulation of CCaMK is essential for symbiotic infection

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