Nanosilver Mitigates Biofilm Formation via FapC Amyloidosis Inhibition

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DOI

  • Zil-E Huma, Department of Chemistry & Chemical Engineering, SBA School of Science & Engineering (SBASSE), Lahore University of Management Science (LUMS), DHA, Lahore, 54792, Pakistan.
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  • Ibrahim Javed, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD, 4072, Australia.
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  • Zhenzhen Zhang, Department of Physics and Astronomy, Clemson University, Clemson, SC, 29634, USA.
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  • Hajira Bilal, Centre for Medicine Use and Safety, Monash Institute of Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville, VIC, 3052, Australia.
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  • Yunxiang Sun, Department of Physics, Faculty of Science, Ningbo University, Ningbo, 315211, China.
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  • Syed Zajif Hussain, Department of Chemistry & Chemical Engineering, SBA School of Science & Engineering (SBASSE), Lahore University of Management Science (LUMS), DHA, Lahore, 54792, Pakistan.
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  • Thomas P Davis, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD, 4072, Australia.
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  • Daniel E Otzen
  • Cornelia B Landersdorfer, Centre for Medicine Use and Safety, Monash Institute of Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville, VIC, 3052, Australia.
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  • Feng Ding, Department of Physics and Astronomy, Clemson University, Clemson, SC, 29634, USA.
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  • Irshad Hussain, Department of Chemistry & Chemical Engineering, SBA School of Science & Engineering (SBASSE), Lahore University of Management Science (LUMS), DHA, Lahore, 54792, Pakistan.
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  • Pu Chun Ke, ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Monash Institute of Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville, VIC, 3052, Australia.

Multidrug resistance of bacteria is a major challenge due to the wide-spread use of antibiotics. While a range of strategies have been developed in recent years, suppression of bacterial activity and virulence via their network of extracellular amyloid has rarely been explored, especially with nanomaterials. Here, silver nanoparticles and nanoclusters (AgNPs and AgNCs) capped with cationic branched polyethylenimine polymer are synthesized, and their antimicrobial potentials are determined at concentrations safe to mammalian cells. Compared with the ultrasmall AgNCs, AgNPs entail stronger binding to suppress the fibrillization of FapC, a major protein constituent of the extracellular amyloid matrix of Pseudomonas aeruginosa. Both types of nanoparticles exhibit concentration-dependent antibiofilm and antimicrobial properties against P. aeruginosa. At concentrations of 1 × 10-6 m or below, both the bactericidal activity of AgNCs and the antibiofilm capacity of AgNPs are associated with their structure-mediated bio-nano interactions but not ion release. For AgNPs, specifically, their antibiofilm potency correlates with their capacity of FapC fibrillization inhibition, but not with their bactericidal activity. This study demonstrates the antimicrobial potential of safe nanotechnology through the novel route of amyloidosis inhibition.

Original languageEnglish
JournalSmall (Weinheim an der Bergstrasse, Germany)
Pages (from-to)e1906674
ISSN1613-6810
DOIs
Publication statusE-pub ahead of print - 27 Jan 2020
Externally publishedYes

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