Nanoparticle-Based Delivery System for Biomedical Applications of RNAi

Research output: Book/anthology/dissertation/reportPh.D. thesisResearch

  • Chuanxu Yang, Denmark
RNA interference (RNAi) is a post-transcriptional gene silencing process triggered by double-strand RNA, including synthetic short interfering RNA (siRNA) and endogenous microRNA (miRNA). RNAi has attracted great attention for developing a new class of therapeutics, due to its capability to specifically inhibit the expression of virtually any gene with high efficiency, including the “undruggable” targets. To activate the RNAi pathway, siRNA or miRNA molecules must be transported into the cytoplasm of target cells. However, various barriers impede the transportation of these fragile macromolecules by themselves. These nucleic acids are negatively charged large molecules, hence making it difficult to cross the cell membrane. When injected into the bloodstream, they will be rapidly degraded by plasmatic enzyme or eliminated by renal clearance. Thus, delivery carriers are required to protect siRNA/miRNA and transport them to the action site in the target cells. This thesis describes the development of various nanocarriers for siRNA/miRNA delivery and investigate their potential biomedical applications including: anti-inflammation, tissue engineering and cancer
Original languageEnglish
PublisherInterdisciplinary Nanoscience Center (iNANO), Aarhus University
Number of pages198
Publication statusPublished - 2014

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