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Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Standard

Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis. / Rübsam, Henriette; Krönauer, Christina; Abel, Nikolaj B et al.
In: Science (New York, N.Y.), Vol. 379, No. 6629, 01.2023, p. 272-277.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Harvard

Rübsam, H, Krönauer, C, Abel, NB, Ji, H, Lironi, D, Hansen, SB, Nadzieja, M, Kolte, MV, Abel, D, de Jong, N, Madsen, LH, Liu, H, Stougaard, J, Radutoiu, S & Andersen, KR 2023, 'Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis', Science (New York, N.Y.), vol. 379, no. 6629, pp. 272-277. https://doi.org/10.1126/science.ade9204

APA

Rübsam, H., Krönauer, C., Abel, N. B., Ji, H., Lironi, D., Hansen, S. B., Nadzieja, M., Kolte, M. V., Abel, D., de Jong, N., Madsen, L. H., Liu, H., Stougaard, J., Radutoiu, S., & Andersen, K. R. (2023). Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis. Science (New York, N.Y.), 379(6629), 272-277. https://doi.org/10.1126/science.ade9204

CBE

Rübsam H, Krönauer C, Abel NB, Ji H, Lironi D, Hansen SB, Nadzieja M, Kolte MV, Abel D, de Jong N, et al. 2023. Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis. Science (New York, N.Y.). 379(6629):272-277. https://doi.org/10.1126/science.ade9204

MLA

Rübsam, Henriette et al. "Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis". Science (New York, N.Y.). 2023, 379(6629). 272-277. https://doi.org/10.1126/science.ade9204

Vancouver

Rübsam H, Krönauer C, Abel NB, Ji H, Lironi D, Hansen SB et al. Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis. Science (New York, N.Y.). 2023 Jan;379(6629):272-277. doi: 10.1126/science.ade9204

Author

Rübsam, Henriette ; Krönauer, Christina ; Abel, Nikolaj B et al. / Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis. In: Science (New York, N.Y.). 2023 ; Vol. 379, No. 6629. pp. 272-277.

Bibtex

@article{7b9b576920094999930aed86c1d634d2,
title = "Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis",
abstract = "Understanding the composition and activation of multicomponent receptor complexes is a challenge in biology. To address this, we developed a synthetic approach based on nanobodies to drive assembly and activation of cell surface receptors and apply the concept by manipulating receptors that govern plant symbiosis with nitrogen-fixing bacteria. We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection. We find that organogenesis signaling is mediated by the intracellular kinase domains whereas infection requires functional ectodomains. Finally, we identify evolutionarily distant barley receptors that activate root nodule organogenesis, which could enable engineering of biological nitrogen-fixation into cereals.",
keywords = "Cell Membrane/metabolism, Gene Expression Regulation, Plant, Plant Proteins/genetics, Root Nodules, Plant/metabolism, Signal Transduction, Symbiosis/physiology, Single-Domain Antibodies, Medicago truncatula, Lotus, Lipopolysaccharides/metabolism",
author = "Henriette R{\"u}bsam and Christina Kr{\"o}nauer and Abel, {Nikolaj B} and Hongtao Ji and Damiano Lironi and Hansen, {Simon B} and Marcin Nadzieja and Kolte, {Marie V} and D{\"o}rte Abel and {de Jong}, Noor and Madsen, {Lene H} and Huijun Liu and Jens Stougaard and Simona Radutoiu and Andersen, {Kasper R}",
year = "2023",
month = jan,
doi = "10.1126/science.ade9204",
language = "English",
volume = "379",
pages = "272--277",
journal = "Science (New York, N.Y.)",
issn = "0036-8075",
publisher = "AMER ASSOC ADVANCEMENT SCIENCE",
number = "6629",

}

RIS

TY - JOUR

T1 - Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis

AU - Rübsam, Henriette

AU - Krönauer, Christina

AU - Abel, Nikolaj B

AU - Ji, Hongtao

AU - Lironi, Damiano

AU - Hansen, Simon B

AU - Nadzieja, Marcin

AU - Kolte, Marie V

AU - Abel, Dörte

AU - de Jong, Noor

AU - Madsen, Lene H

AU - Liu, Huijun

AU - Stougaard, Jens

AU - Radutoiu, Simona

AU - Andersen, Kasper R

PY - 2023/1

Y1 - 2023/1

N2 - Understanding the composition and activation of multicomponent receptor complexes is a challenge in biology. To address this, we developed a synthetic approach based on nanobodies to drive assembly and activation of cell surface receptors and apply the concept by manipulating receptors that govern plant symbiosis with nitrogen-fixing bacteria. We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection. We find that organogenesis signaling is mediated by the intracellular kinase domains whereas infection requires functional ectodomains. Finally, we identify evolutionarily distant barley receptors that activate root nodule organogenesis, which could enable engineering of biological nitrogen-fixation into cereals.

AB - Understanding the composition and activation of multicomponent receptor complexes is a challenge in biology. To address this, we developed a synthetic approach based on nanobodies to drive assembly and activation of cell surface receptors and apply the concept by manipulating receptors that govern plant symbiosis with nitrogen-fixing bacteria. We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection. We find that organogenesis signaling is mediated by the intracellular kinase domains whereas infection requires functional ectodomains. Finally, we identify evolutionarily distant barley receptors that activate root nodule organogenesis, which could enable engineering of biological nitrogen-fixation into cereals.

KW - Cell Membrane/metabolism

KW - Gene Expression Regulation, Plant

KW - Plant Proteins/genetics

KW - Root Nodules, Plant/metabolism

KW - Signal Transduction

KW - Symbiosis/physiology

KW - Single-Domain Antibodies

KW - Medicago truncatula

KW - Lotus

KW - Lipopolysaccharides/metabolism

U2 - 10.1126/science.ade9204

DO - 10.1126/science.ade9204

M3 - Journal article

C2 - 36656954

VL - 379

SP - 272

EP - 277

JO - Science (New York, N.Y.)

JF - Science (New York, N.Y.)

SN - 0036-8075

IS - 6629

ER -