Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis

Henriette Rübsam; Christina Krönauer; Nikolaj B Abel; Hongtao Ji; Damiano Lironi; Simon B Hansen; Marcin Nadzieja; Marie V Kolte; Dörte Abel; Noor de Jong; Lene H Madsen; Huijun Liu; Jens Stougaard; Simona Radutoiu; Kasper R Andersen

doi:10.1126/science.ade9204

Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis

Henriette Rübsam, Christina Krönauer, Nikolaj B Abel, Hongtao Ji, Damiano Lironi, Simon B Hansen, Marcin Nadzieja, Marie V Kolte, Dörte Abel, Noor de Jong, Lene H Madsen, Huijun Liu, Jens Stougaard, Simona Radutoiu, Kasper R Andersen

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

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Abstract

Understanding the composition and activation of multicomponent receptor complexes is a challenge in biology. To address this, we developed a synthetic approach based on nanobodies to drive assembly and activation of cell surface receptors and apply the concept by manipulating receptors that govern plant symbiosis with nitrogen-fixing bacteria. We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection. We find that organogenesis signaling is mediated by the intracellular kinase domains whereas infection requires functional ectodomains. Finally, we identify evolutionarily distant barley receptors that activate root nodule organogenesis, which could enable engineering of biological nitrogen-fixation into cereals.

Original language	English
Journal	Science (New York, N.Y.)
Volume	379
Issue	6629
Pages (from-to)	272-277
Number of pages	6
ISSN	0036-8075
DOIs	https://doi.org/10.1126/science.ade9204
Publication status	Published - Jan 2023

Keywords

Cell Membrane/metabolism
Gene Expression Regulation, Plant
Plant Proteins/genetics
Root Nodules, Plant/metabolism
Signal Transduction
Symbiosis/physiology
Single-Domain Antibodies
Medicago truncatula
Lotus
Lipopolysaccharides/metabolism

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Rubsam, H. et al. (2023) Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosisFinal published version, 845 KB

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Rübsam, H., Krönauer, C., Abel, N. B., Ji, H., Lironi, D., Hansen, S. B., Nadzieja, M., Kolte, M. V., Abel, D., de Jong, N., Madsen, L. H., Liu, H., Stougaard, J., Radutoiu, S., & Andersen, K. R. (2023). Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis. Science (New York, N.Y.), 379(6629), 272-277. https://doi.org/10.1126/science.ade9204

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abstract = "Understanding the composition and activation of multicomponent receptor complexes is a challenge in biology. To address this, we developed a synthetic approach based on nanobodies to drive assembly and activation of cell surface receptors and apply the concept by manipulating receptors that govern plant symbiosis with nitrogen-fixing bacteria. We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection. We find that organogenesis signaling is mediated by the intracellular kinase domains whereas infection requires functional ectodomains. Finally, we identify evolutionarily distant barley receptors that activate root nodule organogenesis, which could enable engineering of biological nitrogen-fixation into cereals.",

keywords = "Cell Membrane/metabolism, Gene Expression Regulation, Plant, Plant Proteins/genetics, Root Nodules, Plant/metabolism, Signal Transduction, Symbiosis/physiology, Single-Domain Antibodies, Medicago truncatula, Lotus, Lipopolysaccharides/metabolism",

author = "Henriette R{\"u}bsam and Christina Kr{\"o}nauer and Abel, {Nikolaj B} and Hongtao Ji and Damiano Lironi and Hansen, {Simon B} and Marcin Nadzieja and Kolte, {Marie V} and D{\"o}rte Abel and {de Jong}, Noor and Madsen, {Lene H} and Huijun Liu and Jens Stougaard and Simona Radutoiu and Andersen, {Kasper R}",

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Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis. / Rübsam, Henriette; Krönauer, Christina; Abel, Nikolaj B et al.
In: Science (New York, N.Y.), Vol. 379, No. 6629, 01.2023, p. 272-277.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis

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AU - Krönauer, Christina

AU - Abel, Nikolaj B

AU - Ji, Hongtao

AU - Lironi, Damiano

AU - Hansen, Simon B

AU - Nadzieja, Marcin

AU - Kolte, Marie V

AU - Abel, Dörte

AU - de Jong, Noor

AU - Madsen, Lene H

AU - Liu, Huijun

AU - Stougaard, Jens

AU - Radutoiu, Simona

AU - Andersen, Kasper R

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AB - Understanding the composition and activation of multicomponent receptor complexes is a challenge in biology. To address this, we developed a synthetic approach based on nanobodies to drive assembly and activation of cell surface receptors and apply the concept by manipulating receptors that govern plant symbiosis with nitrogen-fixing bacteria. We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection. We find that organogenesis signaling is mediated by the intracellular kinase domains whereas infection requires functional ectodomains. Finally, we identify evolutionarily distant barley receptors that activate root nodule organogenesis, which could enable engineering of biological nitrogen-fixation into cereals.

KW - Cell Membrane/metabolism

KW - Gene Expression Regulation, Plant

KW - Plant Proteins/genetics

KW - Root Nodules, Plant/metabolism

KW - Signal Transduction

KW - Symbiosis/physiology

KW - Single-Domain Antibodies

KW - Medicago truncatula

KW - Lotus

KW - Lipopolysaccharides/metabolism

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JO - Science (New York, N.Y.)

JF - Science (New York, N.Y.)

IS - 6629

ER -

Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis

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Keywords

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