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Naked siLNA-mediated gene silencing of lung bronchoepithelium EGFP expression after intravenous administration

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  • Department of Medical Microbiology and Immunology
  • iNano-School
  • Department of Molecular Biology
  • Department of Molecular Biology
  • Stereological Research Laboratory
The use of systemic siRNA therapeutics for RNA interference-mediated silencing of disease genes is limited by serum instability and inadequate biodistribution. We have previously reported on the EGFP gene silencing effect of chitosan/siRNA nanoparticles in the bronchoepithelium of mice lungs following intranasal delivery and improved serum stability and reduced off-targeting effects in vitro by incorporation of locked nucleic acid (LNA). In this study, we examine the pulmonary gene silencing effect of siLNAs targeting enhanced-green-fluorescent-protein (EGFP) in lung bronchoepithelium upon intravenous delivery of naked siLNAs and upon intranasal delivery of either naked siLNA or chitosan/siLNA nanoparticles. We show that naked siLNA administered intravenously efficiently reduces the EGFP protein expression. A similar effect is obtained with intranasal delivery of chitosan nanoparticles containing siLNA whereas intranasally instilled naked siLNA did not cause a knockdown.
Original languageEnglish
JournalOligonucleotides
Volume19
Issue2
Pages (from-to)163-8
Number of pages5
ISSN1545-4576
DOIs
Publication statusPublished - 2009

    Research areas

  • Animals, Bronchi, Chitosan, Drug Carriers, Gene Silencing, Green Fluorescent Proteins, Injections, Intravenous, Mice, Mice, Inbred C57BL, Mice, Transgenic, Nanoparticles, Oligonucleotides, RNA, Small Interfering, Respiratory Mucosa

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