N4-acetylcytidine (ac4C) promotes mRNA localization to stress granules

Pavel Kudrin, Ankita Singh, David Meierhofer, Anna Kuśnierczyk, Ulf Andersson Vang Ørom*

*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Abstract

Stress granules are an integral part of the stress response that are formed from non-translating mRNAs aggregated with proteins. While much is known about stress granules, the factors that drive their mRNA localization are incompletely described. Modification of mRNA can alter the properties of the nucleobases and affect processes such as translation, splicing and localization of individual transcripts. Here, we show that the RNA modification N4-acetylcytidine (ac4C) on mRNA associates with transcripts enriched in stress granules and that stress granule localized transcripts with ac4C are specifically translationally regulated. We also show that ac4C on mRNA can mediate localization of the protein NOP58 to stress granules. Our results suggest that acetylation of mRNA regulates localization of both stress-sensitive transcripts and RNA-binding proteins to stress granules and adds to our understanding of the molecular mechanisms responsible for stress granule formation.

Original languageEnglish
JournalEMBO Reports
Volume25
Issue4
Pages (from-to)1814-1834
Number of pages21
ISSN1469-221X
DOIs
Publication statusPublished - Apr 2024

Keywords

  • mRNA Localization
  • RNA Acetylation
  • Stress Granules
  • Translation
  • RNA-Binding Proteins/metabolism
  • Cytidine/genetics
  • RNA, Messenger/genetics

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