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Muscle Tissue Saturation Compared With Muscle Tissue Perfusion During Low Blood Flows: An Experimental Study

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  • Sisse Anette Thomassen, Aalborg University
  • ,
  • Benedict Kjærgaard, Aalborg University
  • ,
  • Aage Kristian Olsen Alstrup
  • Ole Lajord Munk
  • Jørgen Frøkiær
  • Anders Larsson, Hedenstierna Laboratory Department of Surgical Sciences, Uppsala University, Uppsala, Sweden.
  • ,
  • Bodil Steen Rasmussen, Aalborg University

OBJECTIVE: To investigate whether changes in muscle tissue perfusion measured with positron emission tomography would be reflected by parallel changes in muscle tissue oxygen saturation (StO2) measured using near-infrared spectroscopy during high and low blood flow levels achieved using cardiopulmonary bypass (CPB) in an animal model.

DESIGN: A prospective, randomized study.

SETTING: Research laboratory, single institution.

PARTICIPANTS: Eight pigs (69-71 kg).

INTERVENTIONS: In anesthetized pigs, normothermic CPB was established with a blood flow of 60 mL/kg/min for 1 hour. Thereafter, a low blood flow of either 47.5 or 35 mL/kg/min was applied for 1 hour followed by a blood flow of 60 mL/kg/min for an additional hour. Regional StO2 was measured continuously by placing a near-infrared spectroscopy electrode on the skin above the gracilis muscle of the noncannulated back leg. Muscle tissue perfusion was measured using positron emission tomography with (15)O-labeled water during spontaneous circulation and the different CPB blood flows. Systemic oxygen consumption was estimated by measurement of venous saturation and lactate levels.

MEASUREMENTS AND MAIN RESULTS: The results showed profound systemic ischemia during low CPB blood flow. StO2 remained high until muscle tissue perfusion decreased to about 50%, after which StO2 paralleled the linear decrease in muscle tissue perfusion.

CONCLUSION: In an experimental CPB animal model, StO2 was stable until muscle tissue perfusion was reduced by about 50%, and at lower blood flow levels, there was almost a linear relationship between StO2 and muscle tissue perfusion.

Original languageEnglish
JournalJournal of Cardiothoracic and Vascular Anesthesia
Volume31(6)
Pages (from-to)2065-2071
ISSN1053-0770
DOIs
Publication statusPublished - Dec 2017

    Research areas

  • Journal Article

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