Abstract
Jusification
Understanding the structure-function relationship in high moisture extrusion (HME) remains a challenge. This work explored the mechanisms underpinning the interactions between biopolymers by studying the interplay of protein purity, starch concentration, and lamella alignment in HME of plant protein blends.
Objecives
The research objective was to address structuring hypotheses using the most advanced rheological and microstructural tools and contrasng systems comprising pea protein isolate both with and without added starch, against pea protein concentrate and fava bean protein concentrate.
Methods
The study employed a Parallel Twin-screw Extruder to prepare various structuring levels for different blends. Mechanical property evaluations were conducted through Texture Profile Analysis (TPA) and Dynamic Mechanical Analysis (DMA). Microstructural insights were obtained via Confocal laser scanning microscopy (CLSM) and Confocal Raman microscopy, while oscillatory rheology (SAOS and LAOS) assessed nanoscale cross-link density.
Results
Confocal Raman microscopy uniquely revealed phase separation between protein-rich and water-rich domains. CLSM showcased a disruptive impact of the dispersed phase on the continuous protein network. Complementary rheological techniques are required to understand properties across length scales. Mechanical testing unveiled the effects of starch on elasticity and deformation, demonstrating increased internal tension and cross-linking with increased starch content. A notable correlation emerged between solubility and hydration development during extrusion, the total mechanical input (SME), and the velocity profile, collectively influence the obtained structure. Lamella angles exhibited parabolic V-shapes at high SME (264 KJ/kg) and softer U-shapes at lower SME values (188 KJ/kg), establishing a clearer link between process variables and resulting structural outcomes.
Significant of research
This research reveals that a muliscale approach was essential for a comprehensive understanding of the structure. The assessment of structure provides valuable information about predominant bonds, cross-linking in the continuous phase, and the strength of the dispersed phase, allowing for improved design of extruded products.
Understanding the structure-function relationship in high moisture extrusion (HME) remains a challenge. This work explored the mechanisms underpinning the interactions between biopolymers by studying the interplay of protein purity, starch concentration, and lamella alignment in HME of plant protein blends.
Objecives
The research objective was to address structuring hypotheses using the most advanced rheological and microstructural tools and contrasng systems comprising pea protein isolate both with and without added starch, against pea protein concentrate and fava bean protein concentrate.
Methods
The study employed a Parallel Twin-screw Extruder to prepare various structuring levels for different blends. Mechanical property evaluations were conducted through Texture Profile Analysis (TPA) and Dynamic Mechanical Analysis (DMA). Microstructural insights were obtained via Confocal laser scanning microscopy (CLSM) and Confocal Raman microscopy, while oscillatory rheology (SAOS and LAOS) assessed nanoscale cross-link density.
Results
Confocal Raman microscopy uniquely revealed phase separation between protein-rich and water-rich domains. CLSM showcased a disruptive impact of the dispersed phase on the continuous protein network. Complementary rheological techniques are required to understand properties across length scales. Mechanical testing unveiled the effects of starch on elasticity and deformation, demonstrating increased internal tension and cross-linking with increased starch content. A notable correlation emerged between solubility and hydration development during extrusion, the total mechanical input (SME), and the velocity profile, collectively influence the obtained structure. Lamella angles exhibited parabolic V-shapes at high SME (264 KJ/kg) and softer U-shapes at lower SME values (188 KJ/kg), establishing a clearer link between process variables and resulting structural outcomes.
Significant of research
This research reveals that a muliscale approach was essential for a comprehensive understanding of the structure. The assessment of structure provides valuable information about predominant bonds, cross-linking in the continuous phase, and the strength of the dispersed phase, allowing for improved design of extruded products.
| Original language | Danish |
|---|---|
| Publication date | 30 Apr 2024 |
| Publication status | Published - 30 Apr 2024 |
| Event | AOCS Annual Meeting & Expo 2024 - Montréal, Canada Duration: 28 Apr 2024 → 1 May 2024 |
Conference
| Conference | AOCS Annual Meeting & Expo 2024 |
|---|---|
| Country/Territory | Canada |
| City | Montréal |
| Period | 28/04/2024 → 01/05/2024 |