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Multiple-timescale dynamics of side-chain carboxyl and carbonyl groups in proteins by 13C nuclear spin relaxation

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  • Raphaël Paquin, École Normale Supérieure, Unknown
  • Fabien Ferrage, École Normale Supérieure, Unknown
  • Frans A A Mulder
  • Mikael Akke, Lund University, Sweden
  • Geoffrey Bodenhausen, École Normale Supérieure, Unknown

Side-chain carboxyl and carbonyl groups play a major role in protein interactions and enzyme catalysis. A series of 13C relaxation experiments is introduced to study the dynamics of carboxyl and carbonyl groups in protein side chains on both fast (sub-ns) and slower (μs-ms) time scales. This approach is illustrated on the protein calbindin D9k. Fast dynamics features correlate with hydrogen- and ion-binding patterns. We also identify chemical dynamics on μs time scales in solvent-exposed carboxyl groups, most probably due to exchange between the carboxylate and carboxylic acid forms.

Original languageEnglish
JournalJournal of the American Chemical Society
Pages (from-to)15805-15807
Number of pages3
Publication statusPublished - 26 Nov 2008
Externally publishedYes

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