TY - JOUR
T1 - Multinuclear MRI Can Depict Metabolic and Energetic Changes in Mild Traumatic Brain Injury
AU - Thorsen, Thomas M
AU - Bøgh, Nikolaj
AU - Bertelsen, Lotte B
AU - Hansen, Esben S S
AU - Laustsen, Christoffer
N1 - © 2024 The Author(s). NMR in Biomedicine published by John Wiley & Sons Ltd.
PY - 2025/1
Y1 - 2025/1
N2 - Mild traumatic brain injuries (TBIs) are frequent in the European population. The pathophysiological changes after TBI include metabolic changes, but these are not observable using current clinical tools. We aimed to evaluate multinuclear MRI as a mean of assessing these changes. In our model, pigs were exposed to a controlled cortical impact (CCI) directly on the dura and scanned at 2 h and 2 days after injury. A multinuclear MRI protocol was used. It included hyperpolarized [1-
13C]pyruvate MRI, which allows depiction of hyperpolarized carbon-13, through its metabolism from pyruvate to lactate or bicarbonate. At Day 2, cerebral microdialysis were performed, and tissue was obtained for analyses. At Day 0, the cerebral blood flow was reduced in the affected hemisphere (TBI: 31.7 mL/100 mL/min, contralateral: 35.6 mL/100 mL/min, p = 0.1227), and the impacted area showed reduced oxygenation (R
2*, TBI: 33.11 s
-1, contralateral: 22.20 s
-1, p = 0.035). At both days, the lactate-to-pyruvate ratios (hyperpolarized [1-
13C]pyruvate) were increased (Day 0: p = 0.023, Day 2: p = 0.022). However, this study can only evaluate the total injury and, thus, cannot differentiate effects from craniotomy and CCI. This metabolic difference was not found using cerebral microdialysis nor a lactate dehydrogenase (LDH) activity assay. The metabolic changes depicted in this study contributes to our understanding of mild TBI; however, the clinical potential of multinuclear MRI is yet to be determined.
AB - Mild traumatic brain injuries (TBIs) are frequent in the European population. The pathophysiological changes after TBI include metabolic changes, but these are not observable using current clinical tools. We aimed to evaluate multinuclear MRI as a mean of assessing these changes. In our model, pigs were exposed to a controlled cortical impact (CCI) directly on the dura and scanned at 2 h and 2 days after injury. A multinuclear MRI protocol was used. It included hyperpolarized [1-
13C]pyruvate MRI, which allows depiction of hyperpolarized carbon-13, through its metabolism from pyruvate to lactate or bicarbonate. At Day 2, cerebral microdialysis were performed, and tissue was obtained for analyses. At Day 0, the cerebral blood flow was reduced in the affected hemisphere (TBI: 31.7 mL/100 mL/min, contralateral: 35.6 mL/100 mL/min, p = 0.1227), and the impacted area showed reduced oxygenation (R
2*, TBI: 33.11 s
-1, contralateral: 22.20 s
-1, p = 0.035). At both days, the lactate-to-pyruvate ratios (hyperpolarized [1-
13C]pyruvate) were increased (Day 0: p = 0.023, Day 2: p = 0.022). However, this study can only evaluate the total injury and, thus, cannot differentiate effects from craniotomy and CCI. This metabolic difference was not found using cerebral microdialysis nor a lactate dehydrogenase (LDH) activity assay. The metabolic changes depicted in this study contributes to our understanding of mild TBI; however, the clinical potential of multinuclear MRI is yet to be determined.
KW - Animals
KW - Brain Concussion/diagnostic imaging
KW - Brain Injuries, Traumatic/diagnostic imaging
KW - Cerebrovascular Circulation
KW - Energy Metabolism
KW - Female
KW - Lactic Acid/metabolism
KW - Magnetic Resonance Imaging
KW - Male
KW - Microdialysis
KW - Pyruvic Acid/metabolism
KW - Swine
UR - http://www.scopus.com/inward/record.url?scp=85212152887&partnerID=8YFLogxK
U2 - 10.1002/nbm.5306
DO - 10.1002/nbm.5306
M3 - Journal article
C2 - 39676068
SN - 0952-3480
VL - 38
SP - e5306
JO - NMR in Biomedicine
JF - NMR in Biomedicine
IS - 1
M1 - e5306
ER -