Multicompartmentalized Microreactors Containing Nuclei and Catalase-Loaded Liposomes

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Multicompartmentalized Microreactors Containing Nuclei and Catalase-Loaded Liposomes. / Zhu, Chuntao; Itel, Fabian; Chandrawati, Rona; Han, Xiaojun; Stadler, Brigitte.

In: Biomacromolecules, Vol. 19, No. 11, 12.11.2018, p. 4379-4385.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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Zhu, C, Itel, F, Chandrawati, R, Han, X & Stadler, B 2018, 'Multicompartmentalized Microreactors Containing Nuclei and Catalase-Loaded Liposomes', Biomacromolecules, vol. 19, no. 11, pp. 4379-4385. https://doi.org/10.1021/acs.biomac.8b01260

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Author

Zhu, Chuntao ; Itel, Fabian ; Chandrawati, Rona ; Han, Xiaojun ; Stadler, Brigitte. / Multicompartmentalized Microreactors Containing Nuclei and Catalase-Loaded Liposomes. In: Biomacromolecules. 2018 ; Vol. 19, No. 11. pp. 4379-4385.

Bibtex

@article{4488a0990cc64459baefc26b139c4f9d,
title = "Multicompartmentalized Microreactors Containing Nuclei and Catalase-Loaded Liposomes",
abstract = "Multicompartmentalized microreactors are considered as cell mimics with hierarchical structures inspired by mammalian cells. We report the successful assembly and encapsulation of purified nuclei from RAW 264.7 cells (pNuc) into alginate-based micro reactors. We demonstrate the preserved function of nuclei within the microreactors for mRNA production. Further, we load catalase-loaded liposomes (Lcat) into the microreactors to break down hydrogen peroxide (H2O2) into oxygen and water. Assemblies containing both natural pNuc and synthetic Lcat show significantly higher mRNA production in the presence of H2O2 compared to microreactors without Lcat or no H2O2 present, suggesting a beneficial effect of the locally enzymatically produced oxygen for transcription. This novel type of microreactors, containing both natural and synthetic compartments, presents a substantial advancement from assemblies equipped with solely synthetic units and offers opportunities as hypoxia models or for cell-free protein synthesis.",
keywords = "MESSENGER-RNA TRANSLATION, ARTIFICIAL CELLS, HYDROGEN-PEROXIDE, MAMMALIAN-CELLS, ALPHA-AMANITIN, ALGINATE BEADS, DRUG CARRIERS, LIVING CELLS, ENCAPSULATION, DNA",
author = "Chuntao Zhu and Fabian Itel and Rona Chandrawati and Xiaojun Han and Brigitte Stadler",
year = "2018",
month = nov,
day = "12",
doi = "10.1021/acs.biomac.8b01260",
language = "English",
volume = "19",
pages = "4379--4385",
journal = "Biomacromolecules",
issn = "1525-7797",
publisher = "AMER CHEMICAL SOC",
number = "11",

}

RIS

TY - JOUR

T1 - Multicompartmentalized Microreactors Containing Nuclei and Catalase-Loaded Liposomes

AU - Zhu, Chuntao

AU - Itel, Fabian

AU - Chandrawati, Rona

AU - Han, Xiaojun

AU - Stadler, Brigitte

PY - 2018/11/12

Y1 - 2018/11/12

N2 - Multicompartmentalized microreactors are considered as cell mimics with hierarchical structures inspired by mammalian cells. We report the successful assembly and encapsulation of purified nuclei from RAW 264.7 cells (pNuc) into alginate-based micro reactors. We demonstrate the preserved function of nuclei within the microreactors for mRNA production. Further, we load catalase-loaded liposomes (Lcat) into the microreactors to break down hydrogen peroxide (H2O2) into oxygen and water. Assemblies containing both natural pNuc and synthetic Lcat show significantly higher mRNA production in the presence of H2O2 compared to microreactors without Lcat or no H2O2 present, suggesting a beneficial effect of the locally enzymatically produced oxygen for transcription. This novel type of microreactors, containing both natural and synthetic compartments, presents a substantial advancement from assemblies equipped with solely synthetic units and offers opportunities as hypoxia models or for cell-free protein synthesis.

AB - Multicompartmentalized microreactors are considered as cell mimics with hierarchical structures inspired by mammalian cells. We report the successful assembly and encapsulation of purified nuclei from RAW 264.7 cells (pNuc) into alginate-based micro reactors. We demonstrate the preserved function of nuclei within the microreactors for mRNA production. Further, we load catalase-loaded liposomes (Lcat) into the microreactors to break down hydrogen peroxide (H2O2) into oxygen and water. Assemblies containing both natural pNuc and synthetic Lcat show significantly higher mRNA production in the presence of H2O2 compared to microreactors without Lcat or no H2O2 present, suggesting a beneficial effect of the locally enzymatically produced oxygen for transcription. This novel type of microreactors, containing both natural and synthetic compartments, presents a substantial advancement from assemblies equipped with solely synthetic units and offers opportunities as hypoxia models or for cell-free protein synthesis.

KW - MESSENGER-RNA TRANSLATION

KW - ARTIFICIAL CELLS

KW - HYDROGEN-PEROXIDE

KW - MAMMALIAN-CELLS

KW - ALPHA-AMANITIN

KW - ALGINATE BEADS

KW - DRUG CARRIERS

KW - LIVING CELLS

KW - ENCAPSULATION

KW - DNA

U2 - 10.1021/acs.biomac.8b01260

DO - 10.1021/acs.biomac.8b01260

M3 - Journal article

C2 - 30231209

VL - 19

SP - 4379

EP - 4385

JO - Biomacromolecules

JF - Biomacromolecules

SN - 1525-7797

IS - 11

ER -