Multicompartmentalized Microreactors Containing Nuclei and Catalase-Loaded Liposomes

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  • Chuntao Zhu, Harbin Institute of Technology
  • ,
  • Fabian Itel
  • ,
  • Rona Chandrawati, The University of New South Wales
  • ,
  • Xiaojun Han, Harbin Institute of Technology
  • ,
  • Brigitte Stadler

Multicompartmentalized microreactors are considered as cell mimics with hierarchical structures inspired by mammalian cells. We report the successful assembly and encapsulation of purified nuclei from RAW 264.7 cells (pNuc) into alginate-based micro reactors. We demonstrate the preserved function of nuclei within the microreactors for mRNA production. Further, we load catalase-loaded liposomes (Lcat) into the microreactors to break down hydrogen peroxide (H2O2) into oxygen and water. Assemblies containing both natural pNuc and synthetic Lcat show significantly higher mRNA production in the presence of H2O2 compared to microreactors without Lcat or no H2O2 present, suggesting a beneficial effect of the locally enzymatically produced oxygen for transcription. This novel type of microreactors, containing both natural and synthetic compartments, presents a substantial advancement from assemblies equipped with solely synthetic units and offers opportunities as hypoxia models or for cell-free protein synthesis.

Original languageEnglish
JournalBiomacromolecules
Volume19
Issue11
Pages (from-to)4379-4385
Number of pages7
ISSN1525-7797
DOIs
Publication statusPublished - 12 Nov 2018

    Research areas

  • MESSENGER-RNA TRANSLATION, ARTIFICIAL CELLS, HYDROGEN-PEROXIDE, MAMMALIAN-CELLS, ALPHA-AMANITIN, ALGINATE BEADS, DRUG CARRIERS, LIVING CELLS, ENCAPSULATION, DNA

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