TY - JOUR
T1 - mRNA and Protein Expression in Human Fetal Membrane Cells
T2 - Potential Biomarkers for Preterm Prelabor Rupture of the Fetal Membranes?
AU - Mikkelsen, Emmeli Fredsgaard R
AU - Huppertz, Berthold
AU - Singh, Ripudaman
AU - Ravn, Katarina
AU - Hatt, Lotte
AU - Kruhøffer, Mogens
AU - Urrabaz-Garza, Rheanna
AU - Uldbjerg, Niels
AU - Menon, Ramkumar
AU - Steiniche, Torben
PY - 2023/11
Y1 - 2023/11
N2 - Clinically, unique markers in fetal membrane cells may contribute to the search for biomarkers for preterm prelabor rupture of the fetal membranes (pPROM) in maternal blood. pPROM is associated with overwhelming inflammation and premature cellular senescence causing “biological microfractures” of the fetal membranes. We hypothesize that these pathological processes are associated with the shedding of fetal membrane cells into the maternal circulation. The aim of this study was to identify markers expressed exclusively in fetal membrane cells to facilitate their isolation, characterization, and determination of biomarker potential in maternal blood. We have (1), by their transcriptomic profile, identified markers that are upregulated in amnion and chorion tissue compared to maternal white blood cells, and (2), by immunohistochemistry, confirmed the localization of the differentially expressed proteins in fetal membranes, placenta, and the placental bed of the uterus. RNA sequencing revealed 31 transcripts in the amnion and 42 transcripts in the chorion that were upregulated. Among these, 22 proteins were evaluated by immunohistochemistry. All but two transcripts were expressed both on mRNA and protein level in at least one fetal membrane cell type. Among these remaining 20 proteins, 9 proteins were not significantly expressed in the villous and extravillous trophoblasts of the placenta.
AB - Clinically, unique markers in fetal membrane cells may contribute to the search for biomarkers for preterm prelabor rupture of the fetal membranes (pPROM) in maternal blood. pPROM is associated with overwhelming inflammation and premature cellular senescence causing “biological microfractures” of the fetal membranes. We hypothesize that these pathological processes are associated with the shedding of fetal membrane cells into the maternal circulation. The aim of this study was to identify markers expressed exclusively in fetal membrane cells to facilitate their isolation, characterization, and determination of biomarker potential in maternal blood. We have (1), by their transcriptomic profile, identified markers that are upregulated in amnion and chorion tissue compared to maternal white blood cells, and (2), by immunohistochemistry, confirmed the localization of the differentially expressed proteins in fetal membranes, placenta, and the placental bed of the uterus. RNA sequencing revealed 31 transcripts in the amnion and 42 transcripts in the chorion that were upregulated. Among these, 22 proteins were evaluated by immunohistochemistry. All but two transcripts were expressed both on mRNA and protein level in at least one fetal membrane cell type. Among these remaining 20 proteins, 9 proteins were not significantly expressed in the villous and extravillous trophoblasts of the placenta.
KW - RNA sequencing
KW - amniochorionic membranes
KW - biomarkers
KW - fetal membranes
KW - immunohistochemistry
KW - mRNA expression
KW - placenta
KW - placental bed
KW - preterm prelabor rupture of membranes
KW - protein expression
U2 - 10.3390/ijms242115826
DO - 10.3390/ijms242115826
M3 - Journal article
C2 - 37958809
SN - 1661-6596
VL - 24
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 21
M1 - 15826
ER -