Movies of ice-embedded particles enhance resolution in electron cryo-microscopy

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • Melody G Campbell, National Resource for Automated Molecular Microscopy, Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA., Denmark
  • Anchi Cheng, Denmark
  • Axel F Brilot, Denmark
  • Arne Möller, National Resource for Automated Molecular Microscopy, Department of Cell Biology, The Scripps Research Institute, La Jolla, California, USA., Denmark
  • Dmitry Lyumkis, Denmark
  • David Veesler, Denmark
  • Junhua Pan, Denmark
  • Stephen C Harrison, Denmark
  • Clinton S Potter
  • ,
  • Bridget Carragher
  • ,
  • Nikolaus Grigorieff, Denmark

Low-dose images obtained by electron cryo-microscopy (cryo-EM) are often affected by blurring caused by sample motion during electron beam exposure, degrading signal especially at high resolution. We show here that we can align frames of movies, recorded with a direct electron detector during beam exposure of rotavirus double-layered particles, thereby greatly reducing image blurring caused by beam-induced motion and sample stage instabilities. This procedure increases the efficiency of cryo-EM imaging and enhances the resolution obtained in three-dimensional reconstructions of the particle. Using movies in this way is generally applicable to all cryo-EM samples and should also improve the performance of midrange electron microscopes that may have limited mechanical stability and beam coherence.

Original languageEnglish
JournalStructure
Volume20
Issue11
Pages (from-to)1823-8
Number of pages6
ISSN0910-8270
DOIs
Publication statusPublished - 7 Nov 2012
Externally publishedYes

    Research areas

  • Cryoelectron Microscopy, Ice, Rotavirus

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