Monitoring soil bacteria with community-level physiological profiles using Biolog™ ECO-plates in the Netherlands and Europe

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  • Michiel Rutgers, National Institute for Public Health and the Environment (RIVM), Bilthoven
  • ,
  • Marja Wouterse, National Institute for Public Health and the Environment (RIVM), Bilthoven, Denmark
  • Sytske M. Drost, National Institute for Public Health and the Environment (RIVM), Bilthoven, Denmark
  • Anton M. Breure, National Institute for Public Health and the Environment (RIVM), Department of Environmental Science, Institute for Water and Wetland Research (IWWR), Radboud University Nijmegen, Denmark
  • Christian Mulder, National Institute for Public Health and the Environment (RIVM), Bilthoven
  • ,
  • Dorothy Stone, Soils and Land Use Research Department, Leeds University, United Kingdom
  • Rachel E. Creamer, Soils and Land Use Research Department, Denmark
  • Anne Winding
  • Jaap Bloem, Wageningen University and Research Centre
Soil samples were analyzed with community-level physiological profiles (CLPP) using Biolog™ ECO-plates in the Netherlands Soil Monitoring Network (NSMN; 704 samples) and in a European-wide transect (73 samples). The selection of sites was based on a representative sample of major soil texture types, land uses and climate zones. This method for determination of CLPP was specifically designed to be applied in large and long-term monitoring activities. It encompassed a solution for the inoculum-density dependence of colour formation patterns in ECO-plates, through stepwise dilution to extinction of the bacterial inoculum. The CLPP in Dutch and European soil samples appeared to be reproducible and sensitive to land use and/or soil texture. Although the method is selective, CLPP based parameters correlated well with other microbial parameters and soil characteristics. Consistent patterns in CLPP and soil habitat characteristics are emerging, as brought about by environmental disturbances, land management and soil texture. The applicability of CLPP analysis in monitoring systems is discussed.
Original languageEnglish
JournalApplied Soil Ecology
Volume97
Pages (from-to)23–35
Number of pages13
ISSN0929-1393
DOIs
Publication statusPublished - Jan 2016

    Research areas

  • Microbial community, BIOLOG, MicroResp, Soil, Indicator, Method

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