Monitoring patterned enzymatic polymerization on DNA origami at single-molecule level

Anders Hauge Okholm; Hüsnü Aslan; Flemming Besenbacher; MD Dong; Jørgen Kjems

doi:10.1039/c5nr01945a

Monitoring patterned enzymatic polymerization on DNA origami at single-molecule level

Anders Hauge Okholm, Hüsnü Aslan, Flemming Besenbacher, MD Dong, Jørgen Kjems

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

13 Citations (Scopus)

Abstract

DNA origami has been used to orchestrate reactions with nano-precision using a variety of biomolecules. Here, the dynamics of albumin-assisted, localized single-molecule DNA polymerization by terminal deoxynucleotidyl transferase on a 2D DNA origami are monitored using AFM in liquid. Direct visualization of the surface activity revealed the mechanics of growth.

Original language	English
Journal	Nanoscale
Volume	7
Issue	25
Pages (from-to)	10970-10973
Number of pages	4
ISSN	2040-3364
DOIs	https://doi.org/10.1039/c5nr01945a
Publication status	Published - 7 Jul 2015

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title = "Monitoring patterned enzymatic polymerization on DNA origami at single-molecule level",

abstract = "DNA origami has been used to orchestrate reactions with nano-precision using a variety of biomolecules. Here, the dynamics of albumin-assisted, localized single-molecule DNA polymerization by terminal deoxynucleotidyl transferase on a 2D DNA origami are monitored using AFM in liquid. Direct visualization of the surface activity revealed the mechanics of growth.",

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T1 - Monitoring patterned enzymatic polymerization on DNA origami at single-molecule level

AU - Okholm, Anders Hauge

AU - Aslan, Hüsnü

AU - Besenbacher, Flemming

AU - Dong, MD

AU - Kjems, Jørgen

PY - 2015/7/7

Y1 - 2015/7/7

N2 - DNA origami has been used to orchestrate reactions with nano-precision using a variety of biomolecules. Here, the dynamics of albumin-assisted, localized single-molecule DNA polymerization by terminal deoxynucleotidyl transferase on a 2D DNA origami are monitored using AFM in liquid. Direct visualization of the surface activity revealed the mechanics of growth.

AB - DNA origami has been used to orchestrate reactions with nano-precision using a variety of biomolecules. Here, the dynamics of albumin-assisted, localized single-molecule DNA polymerization by terminal deoxynucleotidyl transferase on a 2D DNA origami are monitored using AFM in liquid. Direct visualization of the surface activity revealed the mechanics of growth.

U2 - 10.1039/c5nr01945a

DO - 10.1039/c5nr01945a

M3 - Journal article

C2 - 26061114

SN - 2040-3364

VL - 7

SP - 10970

EP - 10973

JO - Nanoscale

JF - Nanoscale

IS - 25

ER -

Monitoring patterned enzymatic polymerization on DNA origami at single-molecule level

Abstract

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