Molecular imprinting of complex matrices at localized surface plasmon resonance biosensors for screening of global interactions of polyphenols and proteins

Joana Rafaela Guerreiro; Vladimir Bochenkov; Kasper Savskov Runager; Hüsnü Aslan; Mingdong Dong; Jan J. Enghild; Victor De Freitas; Maria Goreti Ferreira Sales; Duncan Sutherland

doi:10.1021/acssensors.5b00054

Molecular imprinting of complex matrices at localized surface plasmon resonance biosensors for screening of global interactions of polyphenols and proteins

Joana Rafaela Guerreiro, Vladimir Bochenkov, Kasper Savskov Runager, Hüsnü Aslan, Mingdong Dong, Jan J. Enghild, Victor De Freitas, Maria Goreti Ferreira Sales, Duncan Sutherland

Interdisciplinary Nanoscience Center - INANO-Fysik, iNANO-huset

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

Abstract

Molecular imprinting polymers (MIP) have been applied to capture and stabilize complex protein matrices at plasmonic sensor surfaces. Ultrathin MIP layers at the surface of gold nanodisks enable the label free quantification of global interactions of polyphenols with protein mixtures. Separate polyphenols (catechin, procyanidin B3- catechin dimer, and PGG-pentagalloyl glucose) give specific and different binding levels to the MIP supported saliva plasmonic sensor. The demonstrated biosensor has application to study bioavailability of polyphenols or evaluation of local retention of small drug molecules.

Original language	English
Journal	ACS Sensors
Volume	1
Issue	3
Pages (from-to)	258-264
Number of pages	7
DOIs	https://doi.org/10.1021/acssensors.5b00054
Publication status	Published - 2016

Keywords

biosensing
complex protein matrix
localized surface plasmon resonance (LSPR)
molecular imprinting
nanodisks
polyphenols

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10.1021/acssensors.5b00054

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Guerreiro, J. R., Bochenkov, V., Runager, K. S., Aslan, H., Dong, M., Enghild, J. J., De Freitas, V., Sales, M. G. F., & Sutherland, D. (2016). Molecular imprinting of complex matrices at localized surface plasmon resonance biosensors for screening of global interactions of polyphenols and proteins. ACS Sensors, 1(3), 258-264. https://doi.org/10.1021/acssensors.5b00054

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title = "Molecular imprinting of complex matrices at localized surface plasmon resonance biosensors for screening of global interactions of polyphenols and proteins",

abstract = "Molecular imprinting polymers (MIP) have been applied to capture and stabilize complex protein matrices at plasmonic sensor surfaces. Ultrathin MIP layers at the surface of gold nanodisks enable the label free quantification of global interactions of polyphenols with protein mixtures. Separate polyphenols (catechin, procyanidin B3- catechin dimer, and PGG-pentagalloyl glucose) give specific and different binding levels to the MIP supported saliva plasmonic sensor. The demonstrated biosensor has application to study bioavailability of polyphenols or evaluation of local retention of small drug molecules.",

keywords = "biosensing, complex protein matrix, localized surface plasmon resonance (LSPR), molecular imprinting, nanodisks, polyphenols",

author = "Guerreiro, {Joana Rafaela} and Vladimir Bochenkov and Runager, {Kasper Savskov} and H{\"u}sn{\"u} Aslan and Mingdong Dong and Enghild, {Jan J.} and {De Freitas}, Victor and Sales, {Maria Goreti Ferreira} and Duncan Sutherland",

year = "2016",

doi = "10.1021/acssensors.5b00054",

language = "English",

volume = "1",

pages = "258--264",

journal = "ACS Sensors",

publisher = "AMER CHEMICAL SOC",

number = "3",

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Molecular imprinting of complex matrices at localized surface plasmon resonance biosensors for screening of global interactions of polyphenols and proteins. / Guerreiro, Joana Rafaela; Bochenkov, Vladimir; Runager, Kasper Savskov et al.
In: ACS Sensors, Vol. 1, No. 3, 2016, p. 258-264.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - Molecular imprinting of complex matrices at localized surface plasmon resonance biosensors for screening of global interactions of polyphenols and proteins

AU - Guerreiro, Joana Rafaela

AU - Bochenkov, Vladimir

AU - Runager, Kasper Savskov

AU - Aslan, Hüsnü

AU - Dong, Mingdong

AU - Enghild, Jan J.

AU - De Freitas, Victor

AU - Sales, Maria Goreti Ferreira

AU - Sutherland, Duncan

PY - 2016

Y1 - 2016

N2 - Molecular imprinting polymers (MIP) have been applied to capture and stabilize complex protein matrices at plasmonic sensor surfaces. Ultrathin MIP layers at the surface of gold nanodisks enable the label free quantification of global interactions of polyphenols with protein mixtures. Separate polyphenols (catechin, procyanidin B3- catechin dimer, and PGG-pentagalloyl glucose) give specific and different binding levels to the MIP supported saliva plasmonic sensor. The demonstrated biosensor has application to study bioavailability of polyphenols or evaluation of local retention of small drug molecules.

AB - Molecular imprinting polymers (MIP) have been applied to capture and stabilize complex protein matrices at plasmonic sensor surfaces. Ultrathin MIP layers at the surface of gold nanodisks enable the label free quantification of global interactions of polyphenols with protein mixtures. Separate polyphenols (catechin, procyanidin B3- catechin dimer, and PGG-pentagalloyl glucose) give specific and different binding levels to the MIP supported saliva plasmonic sensor. The demonstrated biosensor has application to study bioavailability of polyphenols or evaluation of local retention of small drug molecules.

KW - biosensing

KW - complex protein matrix

KW - localized surface plasmon resonance (LSPR)

KW - molecular imprinting

KW - nanodisks

KW - polyphenols

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U2 - 10.1021/acssensors.5b00054

DO - 10.1021/acssensors.5b00054

M3 - Journal article

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SP - 258

EP - 264

JO - ACS Sensors

JF - ACS Sensors

IS - 3

ER -

Molecular imprinting of complex matrices at localized surface plasmon resonance biosensors for screening of global interactions of polyphenols and proteins

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Keywords

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