Molecular evidence for sustained transmission of zoonotic Ascaris suum among zoo chimpanzees (Pan troglodytes)

Peter Nejsum; Mads Frost Bertelsen; Martha Betson; Russel Stothard; Darwin Murrell

Abstract

Chimpanzees in the Copenhagen Zoo frequently excrete ascarid worms onto the cage floor inspite of a regular anthelmintic treatment program. Previously it had been shown that the source of the infections was of pig origin. However, it was unknown whether the recurrence of the infection was due to reintroduction of eggs from an external source or to a sustained transmission cycle within the Zoo. We found that isolated eggs were able to embryonate into the infective stage and PCR-RFLP analysis on the ITS region amplified from single embryonated eggs suggest these to be Ascaris suum. In addition, sequence analysis of the cox1 gene (‘barcoding') on expelled worms followed by cluster analysis revealed that the chimpanzees are infected with pig A. suum which now, in spite of control efforts, has stabilized into a permanent transmission cycle in the Zoo's chimpanzee troop

Original language	English
Publication date	2010
Number of pages	1
Publication status	Published - 2010
Externally published	Yes
Event	Joint Spring Symposium of the Danish Society for Parasitology & Danish Society for Tropical Medicine and International Health - Copenhagen, Denmark Duration: 30 Apr 2004 → 30 Apr 2004

Conference

Conference	Joint Spring Symposium of the Danish Society for Parasitology & Danish Society for Tropical Medicine and International Health
Country/Territory	Denmark
City	Copenhagen
Period	30/04/2004 → 30/04/2004

Cite this

@conference{9ec90a2a1d0c4fa8acc5a2edfd95ebd2,

title = "Molecular evidence for sustained transmission of zoonotic Ascaris suum among zoo chimpanzees (Pan troglodytes)",

abstract = "Chimpanzees in the Copenhagen Zoo frequently excrete ascarid worms onto the cage floor inspite of a regular anthelmintic treatment program. Previously it had been shown that the source of the infections was of pig origin. However, it was unknown whether the recurrence of the infection was due to reintroduction of eggs from an external source or to a sustained transmission cycle within the Zoo. We found that isolated eggs were able to embryonate into the infective stage and PCR-RFLP analysis on the ITS region amplified from single embryonated eggs suggest these to be Ascaris suum. In addition, sequence analysis of the cox1 gene ({\textquoteleft}barcoding') on expelled worms followed by cluster analysis revealed that the chimpanzees are infected with pig A. suum which now, in spite of control efforts, has stabilized into a permanent transmission cycle in the Zoo's chimpanzee troop",

author = "Peter Nejsum and Bertelsen, {Mads Frost} and Martha Betson and Russel Stothard and Darwin Murrell",

year = "2010",

language = "English",

note = "Joint Spring Symposium of the Danish Society for Parasitology & Danish Society for Tropical Medicine and International Health ; Conference date: 30-04-2004 Through 30-04-2004",

}

Molecular evidence for sustained transmission of zoonotic Ascaris suum among zoo chimpanzees (Pan troglodytes). / Nejsum, Peter; Bertelsen, Mads Frost; Betson, Martha et al.
2010. Abstract from Joint Spring Symposium of the Danish Society for Parasitology & Danish Society for Tropical Medicine and International Health, Copenhagen, Denmark.

Research output: Contribution to conference › Conference abstract for conference › Research

TY - ABST

T1 - Molecular evidence for sustained transmission of zoonotic Ascaris suum among zoo chimpanzees (Pan troglodytes)

AU - Nejsum, Peter

AU - Bertelsen, Mads Frost

AU - Betson, Martha

AU - Stothard, Russel

AU - Murrell, Darwin

PY - 2010

Y1 - 2010

N2 - Chimpanzees in the Copenhagen Zoo frequently excrete ascarid worms onto the cage floor inspite of a regular anthelmintic treatment program. Previously it had been shown that the source of the infections was of pig origin. However, it was unknown whether the recurrence of the infection was due to reintroduction of eggs from an external source or to a sustained transmission cycle within the Zoo. We found that isolated eggs were able to embryonate into the infective stage and PCR-RFLP analysis on the ITS region amplified from single embryonated eggs suggest these to be Ascaris suum. In addition, sequence analysis of the cox1 gene (‘barcoding') on expelled worms followed by cluster analysis revealed that the chimpanzees are infected with pig A. suum which now, in spite of control efforts, has stabilized into a permanent transmission cycle in the Zoo's chimpanzee troop

AB - Chimpanzees in the Copenhagen Zoo frequently excrete ascarid worms onto the cage floor inspite of a regular anthelmintic treatment program. Previously it had been shown that the source of the infections was of pig origin. However, it was unknown whether the recurrence of the infection was due to reintroduction of eggs from an external source or to a sustained transmission cycle within the Zoo. We found that isolated eggs were able to embryonate into the infective stage and PCR-RFLP analysis on the ITS region amplified from single embryonated eggs suggest these to be Ascaris suum. In addition, sequence analysis of the cox1 gene (‘barcoding') on expelled worms followed by cluster analysis revealed that the chimpanzees are infected with pig A. suum which now, in spite of control efforts, has stabilized into a permanent transmission cycle in the Zoo's chimpanzee troop

M3 - Conference abstract for conference

T2 - Joint Spring Symposium of the Danish Society for Parasitology & Danish Society for Tropical Medicine and International Health

Y2 - 30 April 2004 through 30 April 2004

ER -

Molecular evidence for sustained transmission of zoonotic Ascaris suum among zoo chimpanzees (Pan troglodytes)

Abstract

Conference

Fingerprint

Cite this