Molecular cloning of the gene encoding developing seed l-asparaginase from Lupinus angustifolius

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

  • J M J J Dickson, Denmark
  • Éva Vincze
  • M R Grant, Denmark
  • L A Smith, Denmark
  • K A Rodber, Denmark
  • K J F Farnden, Denmark
  • P H S Reynolds, Denmark
A genomic sequence encoding Lupinus angustifolius L-asparaginase has been obtained, and is the first report of this gene from a plant source. The 3.2 kb of DNA sequenced contains a 1136 bp 5' flanking sequence, four exons and three introns. Intron-exon borders were mapped by comparing the genomic sequence with that of a L. arboreus cDNA. Primer extension analysis revealed transcription start sites 16 bp and 13 bp 5' of the initiating ATG for L. angustifolius and L. arboreus, respectively. The 5' flanking region contained sequences associated with seed-specific expression.
Original languageEnglish
JournalPlant Molecular Biology
Pages (from-to)333-336
Number of pages4
Publication statusPublished - 1992
Externally publishedYes

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