There is immense interest in how the local environment influences the electronic structure of materials at the single-layer limit. We characterize moiré induced spatial variations in the electronic structure of in situ grown monolayer V2S3 on Au(111) by means of low-temperature scanning tunneling microscopy and spectroscopy. We observe a long-range modulation of the integrated local density of states (LDOS), and quantify this modulation with respect to the moiré superstructure for multiple orientations of the monolayer with respect to the substrate. Scanning tunneling spectroscopy reveals a prominent peak in the LDOS, which is shifted in energy at different points of the moiré superstructure. Comparing ab initio calculations with angle-resolved photoemission, we are able to attribute this peak to bands that exhibit a large out of plane d-orbital character. This suggests that the moiré driven variations in the measured density of states are driven by a periodic modulation of the monolayer-substrate hybridization.