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MIRRAGGE - Minimum Information Required for Reproducible AGGregation Experiments

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  • Pedro M Martins, Universidade do Porto
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  • Susanna Navarro, Universitat Autonoma de Barcelona
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  • Alexandra Silva, Universidade do Porto
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  • Maria F Pinto, Universidade do Porto
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  • Zsuzsa Sárkány, Universidade do Porto
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  • Francisco Figueiredo, Universidade do Porto, International Iberian Nanotechnology Laboratory
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  • Pedro José Barbosa Pereira, Universidade do Porto
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  • Francisca Pinheiro, Universitat Autonoma de Barcelona
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  • Zuzana Bednarikova, Slovak Acad Sci, Slovak Academy of Sciences, Inst Bot
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  • Michał Burdukiewicz, Faculty of Physics, Warsaw University of Technology, Warsaw, Poland.
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  • Oxana V Galzitskaya, Russian Acad Sci, Institute of Geography, Russian Academy of Sciences, Russian Academy of Sciences, Inst Geog
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  • Zuzana Gazova, Slovak Acad Sci, Slovak Academy of Sciences, Inst Bot
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  • Cláudio M Gomes, Universidade de Lisboa
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  • Annalisa Pastore, UK-DRI Centre at King's College London
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  • Louise C Serpell, Univ Sussex, University of Sussex, Sch Psychol
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  • Rostislav Skrabana, Slovak Academy of Sciences, AXON Neuroscience CRM Services SE
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  • Vytautas Smirnovas, Vilnius University
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  • Mantas Ziaunys, Vilnius University
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  • Daniel E Otzen
  • Salvador Ventura, Universitat Autonoma de Barcelona
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  • Sandra Macedo-Ribeiro, Universidade do Porto

Reports on phase separation and amyloid formation for multiple proteins and aggregation-prone peptides are recurrently used to explore the molecular mechanisms associated with several human diseases. The information conveyed by these reports can be used directly in translational investigation, e.g., for the design of better drug screening strategies, or be compiled in databases for benchmarking novel aggregation-predicting algorithms. Given that minute protocol variations determine different outcomes of protein aggregation assays, there is a strong urge for standardized descriptions of the different types of aggregates and the detailed methods used in their production. In an attempt to address this need, we assembled the Minimum Information Required for Reproducible Aggregation Experiments (MIRRAGGE) guidelines, considering first-principles and the established literature on protein self-assembly and aggregation. This consensus information aims to cover the major and subtle determinants of experimental reproducibility while avoiding excessive technical details that are of limited practical interest for non-specialized users. The MIRRAGGE table (template available in Supplementary Information) is useful as a guide for the design of new studies and as a checklist during submission of experimental reports for publication. Full disclosure of relevant information also enables other researchers to reproduce results correctly and facilitates systematic data deposition into curated databases.

Original languageEnglish
Article number582488
JournalFrontiers in Molecular Neuroscience
Volume13
Number of pages18
ISSN1662-5099
DOIs
Publication statusPublished - 27 Nov 2020

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