miRNA-dependent gene silencing involving Ago2-mediated cleavage of a circular antisense RNA

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miRNA-dependent gene silencing involving Ago2-mediated cleavage of a circular antisense RNA. / Hansen, Thomas Birkballe; Wiklund, Erik D; Bramsen, Jesper Bertram; Villadsen, Sune B; Statham, Aaron L; Clark, Susan J; Kjems, Jørgen.

In: E M B O Journal, Vol. 30, No. 21, 30.09.2011, p. 4414-4422.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Hansen, TB, Wiklund, ED, Bramsen, JB, Villadsen, SB, Statham, AL, Clark, SJ & Kjems, J 2011, 'miRNA-dependent gene silencing involving Ago2-mediated cleavage of a circular antisense RNA', E M B O Journal, vol. 30, no. 21, pp. 4414-4422. https://doi.org/10.1038/emboj.2011.359

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Vancouver

Hansen TB, Wiklund ED, Bramsen JB, Villadsen SB, Statham AL, Clark SJ et al. miRNA-dependent gene silencing involving Ago2-mediated cleavage of a circular antisense RNA. E M B O Journal. 2011 Sep 30;30(21):4414-4422. https://doi.org/10.1038/emboj.2011.359

Author

Hansen, Thomas Birkballe ; Wiklund, Erik D ; Bramsen, Jesper Bertram ; Villadsen, Sune B ; Statham, Aaron L ; Clark, Susan J ; Kjems, Jørgen. / miRNA-dependent gene silencing involving Ago2-mediated cleavage of a circular antisense RNA. In: E M B O Journal. 2011 ; Vol. 30, No. 21. pp. 4414-4422.

Bibtex

@article{19994e579fc4484691da33635707e3a9,
title = "miRNA-dependent gene silencing involving Ago2-mediated cleavage of a circular antisense RNA",
abstract = "MicroRNAs (miRNAs) are ∼22 nt non-coding RNAs that typically bind to the 3' UTR of target mRNAs in the cytoplasm, resulting in mRNA destabilization and translational repression. Here, we report that miRNAs can also regulate gene expression by targeting non-coding antisense transcripts in human cells. Specifically, we show that miR-671 directs cleavage of a circular antisense transcript of the Cerebellar Degeneration-Related protein 1 (CDR1) locus in an Ago2-slicer-dependent manner. The resulting downregulation of circular antisense has a concomitant decrease in CDR1 mRNA levels, independently of heterochromatin formation. This study provides the first evidence for non-coding antisense transcripts as functional miRNA targets, and a novel regulatory mechanism involving a positive correlation between mRNA and antisense circular RNA levels.",
keywords = "CDR1, circular RNA, miR-671, natural antisense transcript, nuclear microRNA",
author = "Hansen, {Thomas Birkballe} and Wiklund, {Erik D} and Bramsen, {Jesper Bertram} and Villadsen, {Sune B} and Statham, {Aaron L} and Clark, {Susan J} and J{\o}rgen Kjems",
year = "2011",
month = sep,
day = "30",
doi = "10.1038/emboj.2011.359",
language = "English",
volume = "30",
pages = "4414--4422",
journal = "E M B O Journal",
issn = "0261-4189",
publisher = "Wiley-Blackwell Publishing Ltd.",
number = "21",

}

RIS

TY - JOUR

T1 - miRNA-dependent gene silencing involving Ago2-mediated cleavage of a circular antisense RNA

AU - Hansen, Thomas Birkballe

AU - Wiklund, Erik D

AU - Bramsen, Jesper Bertram

AU - Villadsen, Sune B

AU - Statham, Aaron L

AU - Clark, Susan J

AU - Kjems, Jørgen

PY - 2011/9/30

Y1 - 2011/9/30

N2 - MicroRNAs (miRNAs) are ∼22 nt non-coding RNAs that typically bind to the 3' UTR of target mRNAs in the cytoplasm, resulting in mRNA destabilization and translational repression. Here, we report that miRNAs can also regulate gene expression by targeting non-coding antisense transcripts in human cells. Specifically, we show that miR-671 directs cleavage of a circular antisense transcript of the Cerebellar Degeneration-Related protein 1 (CDR1) locus in an Ago2-slicer-dependent manner. The resulting downregulation of circular antisense has a concomitant decrease in CDR1 mRNA levels, independently of heterochromatin formation. This study provides the first evidence for non-coding antisense transcripts as functional miRNA targets, and a novel regulatory mechanism involving a positive correlation between mRNA and antisense circular RNA levels.

AB - MicroRNAs (miRNAs) are ∼22 nt non-coding RNAs that typically bind to the 3' UTR of target mRNAs in the cytoplasm, resulting in mRNA destabilization and translational repression. Here, we report that miRNAs can also regulate gene expression by targeting non-coding antisense transcripts in human cells. Specifically, we show that miR-671 directs cleavage of a circular antisense transcript of the Cerebellar Degeneration-Related protein 1 (CDR1) locus in an Ago2-slicer-dependent manner. The resulting downregulation of circular antisense has a concomitant decrease in CDR1 mRNA levels, independently of heterochromatin formation. This study provides the first evidence for non-coding antisense transcripts as functional miRNA targets, and a novel regulatory mechanism involving a positive correlation between mRNA and antisense circular RNA levels.

KW - CDR1

KW - circular RNA

KW - miR-671

KW - natural antisense transcript

KW - nuclear microRNA

U2 - 10.1038/emboj.2011.359

DO - 10.1038/emboj.2011.359

M3 - Journal article

C2 - 21964070

VL - 30

SP - 4414

EP - 4422

JO - E M B O Journal

JF - E M B O Journal

SN - 0261-4189

IS - 21

ER -