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Minimal DNA requirement for topoisomerase II-mediated cleavage in vitro

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  • K Lund
  • ,
  • A H Andersen
  • K Christiansen
  • ,
  • J Q Svejstrup, Denmark
  • O Westergaard

The minimal DNA requirement for topoisomerase II-mediated DNA cleavage in vitro was determined by analyzing the interaction of the enzyme with sets of DNA substrates varying successively by single bases at the 5'- or 3'-end of either strand. A 16-base pair double-stranded region was established as the minimal duplex region required for topoisomerase II cleavage activity. The region was located symmetrically around the 4-base staggered cleavage site. Topoisomerase II-mediated cleavage within the 16-base pair core duplex, however, required single-stranded regions flanking the duplex to either the 5'- or 3'-sides, or an extension at both ends of the duplex with 1 or more base pairs.

Original languageEnglish
JournalJournal of Biological Chemistry
Volume265
Issue23
Pages (from-to)13856-63
Number of pages8
ISSN0021-9258
Publication statusPublished - 15 Aug 1990

    Research areas

  • Animals, Base Composition, Base Sequence, Cattle, Cloning, Molecular, DNA, DNA Topoisomerases, Type II, Molecular Sequence Data, Oligodeoxyribonucleotides, Substrate Specificity, Thymus Gland, Journal Article, Research Support, Non-U.S. Gov't

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