MicroRNA functionalized microporous titanium oxide surface by lyophilization with enhanced osteogenic activity

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Standard

MicroRNA functionalized microporous titanium oxide surface by lyophilization with enhanced osteogenic activity. / Wu, Kaimin; Song, Wen; Zhao, Lingzhou; Liu, Mengyuan; Yan, Jun; Andersen, Morten Østergaard; Kjems, Jørgen; Gao, Shan; Zhang, Yumei.

In: A C S Applied Materials and Interfaces, Vol. 5, No. 7, 10.04.2013, p. 2733-44.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

Harvard

Wu, K, Song, W, Zhao, L, Liu, M, Yan, J, Andersen, MØ, Kjems, J, Gao, S & Zhang, Y 2013, 'MicroRNA functionalized microporous titanium oxide surface by lyophilization with enhanced osteogenic activity', A C S Applied Materials and Interfaces, vol. 5, no. 7, pp. 2733-44. https://doi.org/10.1021/am400374c

APA

Wu, K., Song, W., Zhao, L., Liu, M., Yan, J., Andersen, M. Ø., Kjems, J., Gao, S., & Zhang, Y. (2013). MicroRNA functionalized microporous titanium oxide surface by lyophilization with enhanced osteogenic activity. A C S Applied Materials and Interfaces, 5(7), 2733-44. https://doi.org/10.1021/am400374c

CBE

Wu K, Song W, Zhao L, Liu M, Yan J, Andersen MØ, Kjems J, Gao S, Zhang Y. 2013. MicroRNA functionalized microporous titanium oxide surface by lyophilization with enhanced osteogenic activity. A C S Applied Materials and Interfaces. 5(7):2733-44. https://doi.org/10.1021/am400374c

MLA

Vancouver

Wu K, Song W, Zhao L, Liu M, Yan J, Andersen MØ et al. MicroRNA functionalized microporous titanium oxide surface by lyophilization with enhanced osteogenic activity. A C S Applied Materials and Interfaces. 2013 Apr 10;5(7):2733-44. https://doi.org/10.1021/am400374c

Author

Wu, Kaimin ; Song, Wen ; Zhao, Lingzhou ; Liu, Mengyuan ; Yan, Jun ; Andersen, Morten Østergaard ; Kjems, Jørgen ; Gao, Shan ; Zhang, Yumei. / MicroRNA functionalized microporous titanium oxide surface by lyophilization with enhanced osteogenic activity. In: A C S Applied Materials and Interfaces. 2013 ; Vol. 5, No. 7. pp. 2733-44.

Bibtex

@article{10fe4aabdca54771b82b637fd5a802a1,
title = "MicroRNA functionalized microporous titanium oxide surface by lyophilization with enhanced osteogenic activity",
abstract = "Developing biomedical titanium (Ti) implants with high osteogenic ability and consequent rigid osseointegration is a constant requirement from the clinic. In this study, we fabricate novel miRNA functionalized microporous Ti implants by lyophilizing miRNA lipoplexes onto a microporous titanium oxide surface formed by microarc oxidation (MAO). The microporous titanium oxide surface provides a larger surface area for miRNA loading and enables spatial retention of the miRNAs within the pores until cellular delivery. The loading of lipoplexes into the micropores on the MAO Ti surface is facilitated by the superhydrophilicity and Ti-OH groups gathering of the MAO surface after UV irradiation followed by lyophilization. A high miRNA transfection efficiency was observed in mesenchymal stem cells (MSCs) seeded onto the miRNA functionalized surface with no apparent cytotoxicity. When functionalizing the Ti surface with miR-29b that enhances osteogenic activity and antimiR-138 that inhibits miR-138 inhibition of endogenous osteogenesis, clear stimulation of MSC osteogenic differentiation was observed, in terms of up-regulating osteogenic expression and enhancing alkaline phosphatase production, collagen secretion and ECM mineralization. The novel miRNA functionalized Ti implants with enhanced osteogenic activity promisingly lead to more rapid and robust osseointegration of a clinical bone implant interface. Our study implies that lyophilization may constitute a versatile method for miRNA loading to other biomaterials with the aim of controlling cellular function.",
author = "Kaimin Wu and Wen Song and Lingzhou Zhao and Mengyuan Liu and Jun Yan and Andersen, {Morten {\O}stergaard} and J{\o}rgen Kjems and Shan Gao and Yumei Zhang",
year = "2013",
month = apr,
day = "10",
doi = "10.1021/am400374c",
language = "English",
volume = "5",
pages = "2733--44",
journal = "A C S Applied Materials and Interfaces",
issn = "1944-8244",
publisher = "American Chemical Society",
number = "7",

}

RIS

TY - JOUR

T1 - MicroRNA functionalized microporous titanium oxide surface by lyophilization with enhanced osteogenic activity

AU - Wu, Kaimin

AU - Song, Wen

AU - Zhao, Lingzhou

AU - Liu, Mengyuan

AU - Yan, Jun

AU - Andersen, Morten Østergaard

AU - Kjems, Jørgen

AU - Gao, Shan

AU - Zhang, Yumei

PY - 2013/4/10

Y1 - 2013/4/10

N2 - Developing biomedical titanium (Ti) implants with high osteogenic ability and consequent rigid osseointegration is a constant requirement from the clinic. In this study, we fabricate novel miRNA functionalized microporous Ti implants by lyophilizing miRNA lipoplexes onto a microporous titanium oxide surface formed by microarc oxidation (MAO). The microporous titanium oxide surface provides a larger surface area for miRNA loading and enables spatial retention of the miRNAs within the pores until cellular delivery. The loading of lipoplexes into the micropores on the MAO Ti surface is facilitated by the superhydrophilicity and Ti-OH groups gathering of the MAO surface after UV irradiation followed by lyophilization. A high miRNA transfection efficiency was observed in mesenchymal stem cells (MSCs) seeded onto the miRNA functionalized surface with no apparent cytotoxicity. When functionalizing the Ti surface with miR-29b that enhances osteogenic activity and antimiR-138 that inhibits miR-138 inhibition of endogenous osteogenesis, clear stimulation of MSC osteogenic differentiation was observed, in terms of up-regulating osteogenic expression and enhancing alkaline phosphatase production, collagen secretion and ECM mineralization. The novel miRNA functionalized Ti implants with enhanced osteogenic activity promisingly lead to more rapid and robust osseointegration of a clinical bone implant interface. Our study implies that lyophilization may constitute a versatile method for miRNA loading to other biomaterials with the aim of controlling cellular function.

AB - Developing biomedical titanium (Ti) implants with high osteogenic ability and consequent rigid osseointegration is a constant requirement from the clinic. In this study, we fabricate novel miRNA functionalized microporous Ti implants by lyophilizing miRNA lipoplexes onto a microporous titanium oxide surface formed by microarc oxidation (MAO). The microporous titanium oxide surface provides a larger surface area for miRNA loading and enables spatial retention of the miRNAs within the pores until cellular delivery. The loading of lipoplexes into the micropores on the MAO Ti surface is facilitated by the superhydrophilicity and Ti-OH groups gathering of the MAO surface after UV irradiation followed by lyophilization. A high miRNA transfection efficiency was observed in mesenchymal stem cells (MSCs) seeded onto the miRNA functionalized surface with no apparent cytotoxicity. When functionalizing the Ti surface with miR-29b that enhances osteogenic activity and antimiR-138 that inhibits miR-138 inhibition of endogenous osteogenesis, clear stimulation of MSC osteogenic differentiation was observed, in terms of up-regulating osteogenic expression and enhancing alkaline phosphatase production, collagen secretion and ECM mineralization. The novel miRNA functionalized Ti implants with enhanced osteogenic activity promisingly lead to more rapid and robust osseointegration of a clinical bone implant interface. Our study implies that lyophilization may constitute a versatile method for miRNA loading to other biomaterials with the aim of controlling cellular function.

U2 - 10.1021/am400374c

DO - 10.1021/am400374c

M3 - Journal article

C2 - 23459382

VL - 5

SP - 2733

EP - 2744

JO - A C S Applied Materials and Interfaces

JF - A C S Applied Materials and Interfaces

SN - 1944-8244

IS - 7

ER -