MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides

Lise M Lindahl; Maria Gluud; Thomas Emmanuel; Emil A Thomsen; Tengpeng Hu; Anne H Rittig; Pamela Celis; Veronica Stolearenco; Thorbjørn Krejsgaard; Claus Johansen; Andreas Willerslev-Olsen; Terkild B Buus; Anders Woetmann; Lars Aagaard; Carsten Geisler; Thomas Litman; Jacob G Mikkelsen; Niels Odum; Lars Iversen

doi:10.2340/00015555-3574

MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides

Lise M Lindahl, Maria Gluud, Thomas Emmanuel, Emil A Thomsen, Tengpeng Hu, Anne H Rittig, Pamela Celis, Veronica Stolearenco, Thorbjørn Krejsgaard, Claus Johansen, Andreas Willerslev-Olsen, Terkild B Buus, Anders Woetmann, Lars Aagaard, Carsten Geisler, Thomas Litman, Jacob G Mikkelsen, Niels Odum, Lars Iversen

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

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Abstract

A prognostic 3-miRNA classifier for early-stage mycosis fungoides has been developed recently, with miR-106b providing the strongest prognostic power. The aim of this study was to investigate the molecular function of miR-106b in mycosis fungoides disease progression. The cellular localization of miR-106b in mycosis fungoides skin biopsies was determined by in situ hybridization. The regulatory role of miR-106b was assessed by transient miR-106b inhibitor/mimic transfection of 2 mycosis fungoides derived cell lines, followed by quantitative real-time PCR (RT-qPCR), western blotting and a proliferation assay. MiR-106b was found to be expressed by dermal T-lymphocytes in mycosis fungoides skin lesions, and miR-106b expression increased with advancing mycosis fungoides stage. Transfection of miR-106b in 2 mycosis fungoides derived cell lines showed that miR-106b represses the tumour suppressors cyclin-dependent kinase inhibitor 1 (p21) and thioredoxin-interacting protein (TXNIP) and promotes mycosis fungoides tumour cell proliferation. In conclusion, these results substantiate that miR-106b has both a functional and prognostic role in progression of mycosis fungoides.

Original language	English
Article number	adv00270
Journal	Acta Dermato-Venereologica
Volume	100
Issue	16
Pages (from-to)	1-8
Number of pages	8
ISSN	0001-5555
DOIs	https://doi.org/10.2340/00015555-3574
Publication status	Published - Sept 2020

Keywords

Cutaneous T-cell lymphoma
MicroRNA
Mycosis fungoides
P21
Progression
TXNIP
Tumour suppressors

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10.2340/00015555-3574

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Lindahl, L. M., Gluud, M., Emmanuel, T., Thomsen, E. A., Hu, T., Rittig, A. H., Celis, P., Stolearenco, V., Krejsgaard, T., Johansen, C., Willerslev-Olsen, A., Buus, T. B., Woetmann, A., Aagaard, L., Geisler, C., Litman, T., Mikkelsen, J. G., Odum, N., & Iversen, L. (2020). MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides. Acta Dermato-Venereologica, 100(16), 1-8. Article adv00270. https://doi.org/10.2340/00015555-3574

@article{a4a34454cdb5453fa50bee456d32b312,

title = "MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides",

abstract = "A prognostic 3-miRNA classifier for early-stage mycosis fungoides has been developed recently, with miR-106b providing the strongest prognostic power. The aim of this study was to investigate the molecular function of miR-106b in mycosis fungoides disease progression. The cellular localization of miR-106b in mycosis fungoides skin biopsies was determined by in situ hybridization. The regulatory role of miR-106b was assessed by transient miR-106b inhibitor/mimic transfection of 2 mycosis fungoides derived cell lines, followed by quantitative real-time PCR (RT-qPCR), western blotting and a proliferation assay. MiR-106b was found to be expressed by dermal T-lymphocytes in mycosis fungoides skin lesions, and miR-106b expression increased with advancing mycosis fungoides stage. Transfection of miR-106b in 2 mycosis fungoides derived cell lines showed that miR-106b represses the tumour suppressors cyclin-dependent kinase inhibitor 1 (p21) and thioredoxin-interacting protein (TXNIP) and promotes mycosis fungoides tumour cell proliferation. In conclusion, these results substantiate that miR-106b has both a functional and prognostic role in progression of mycosis fungoides.",

keywords = "Cutaneous T-cell lymphoma, MicroRNA, Mycosis fungoides, P21, Progression, TXNIP, Tumour suppressors",

author = "Lindahl, {Lise M} and Maria Gluud and Thomas Emmanuel and Thomsen, {Emil A} and Tengpeng Hu and Rittig, {Anne H} and Pamela Celis and Veronica Stolearenco and Thorbj{\o}rn Krejsgaard and Claus Johansen and Andreas Willerslev-Olsen and Buus, {Terkild B} and Anders Woetmann and Lars Aagaard and Carsten Geisler and Thomas Litman and Mikkelsen, {Jacob G} and Niels Odum and Lars Iversen",

year = "2020",

month = sep,

doi = "10.2340/00015555-3574",

language = "English",

volume = "100",

pages = "1--8",

journal = "Acta Dermato-Venereologica",

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Lindahl, LM, Gluud, M, Emmanuel, T , Thomsen, EA, Hu, T, Rittig, AH, Celis, P, Stolearenco, V, Krejsgaard, T, Johansen, C, Willerslev-Olsen, A, Buus, TB, Woetmann, A, Aagaard, L, Geisler, C, Litman, T, Mikkelsen, JG, Odum, N & Iversen, L 2020, 'MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides', Acta Dermato-Venereologica, vol. 100, no. 16, adv00270, pp. 1-8. https://doi.org/10.2340/00015555-3574

MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides. / Lindahl, Lise M; Gluud, Maria; Emmanuel, Thomas et al.
In: Acta Dermato-Venereologica, Vol. 100, No. 16, adv00270, 09.2020, p. 1-8.

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

TY - JOUR

T1 - MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides

AU - Lindahl, Lise M

AU - Gluud, Maria

AU - Emmanuel, Thomas

AU - Thomsen, Emil A

AU - Hu, Tengpeng

AU - Rittig, Anne H

AU - Celis, Pamela

AU - Stolearenco, Veronica

AU - Krejsgaard, Thorbjørn

AU - Johansen, Claus

AU - Willerslev-Olsen, Andreas

AU - Buus, Terkild B

AU - Woetmann, Anders

AU - Aagaard, Lars

AU - Geisler, Carsten

AU - Litman, Thomas

AU - Mikkelsen, Jacob G

AU - Odum, Niels

AU - Iversen, Lars

PY - 2020/9

Y1 - 2020/9

N2 - A prognostic 3-miRNA classifier for early-stage mycosis fungoides has been developed recently, with miR-106b providing the strongest prognostic power. The aim of this study was to investigate the molecular function of miR-106b in mycosis fungoides disease progression. The cellular localization of miR-106b in mycosis fungoides skin biopsies was determined by in situ hybridization. The regulatory role of miR-106b was assessed by transient miR-106b inhibitor/mimic transfection of 2 mycosis fungoides derived cell lines, followed by quantitative real-time PCR (RT-qPCR), western blotting and a proliferation assay. MiR-106b was found to be expressed by dermal T-lymphocytes in mycosis fungoides skin lesions, and miR-106b expression increased with advancing mycosis fungoides stage. Transfection of miR-106b in 2 mycosis fungoides derived cell lines showed that miR-106b represses the tumour suppressors cyclin-dependent kinase inhibitor 1 (p21) and thioredoxin-interacting protein (TXNIP) and promotes mycosis fungoides tumour cell proliferation. In conclusion, these results substantiate that miR-106b has both a functional and prognostic role in progression of mycosis fungoides.

AB - A prognostic 3-miRNA classifier for early-stage mycosis fungoides has been developed recently, with miR-106b providing the strongest prognostic power. The aim of this study was to investigate the molecular function of miR-106b in mycosis fungoides disease progression. The cellular localization of miR-106b in mycosis fungoides skin biopsies was determined by in situ hybridization. The regulatory role of miR-106b was assessed by transient miR-106b inhibitor/mimic transfection of 2 mycosis fungoides derived cell lines, followed by quantitative real-time PCR (RT-qPCR), western blotting and a proliferation assay. MiR-106b was found to be expressed by dermal T-lymphocytes in mycosis fungoides skin lesions, and miR-106b expression increased with advancing mycosis fungoides stage. Transfection of miR-106b in 2 mycosis fungoides derived cell lines showed that miR-106b represses the tumour suppressors cyclin-dependent kinase inhibitor 1 (p21) and thioredoxin-interacting protein (TXNIP) and promotes mycosis fungoides tumour cell proliferation. In conclusion, these results substantiate that miR-106b has both a functional and prognostic role in progression of mycosis fungoides.

KW - Cutaneous T-cell lymphoma

KW - MicroRNA

KW - Mycosis fungoides

KW - P21

KW - Progression

KW - TXNIP

KW - Tumour suppressors

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U2 - 10.2340/00015555-3574

DO - 10.2340/00015555-3574

M3 - Journal article

C2 - 32556351

SN - 0001-5555

VL - 100

SP - 1

EP - 8

JO - Acta Dermato-Venereologica

JF - Acta Dermato-Venereologica

IS - 16

M1 - adv00270

ER -

MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides

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Keywords

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