Micromotor-Assisted Keratinocytes Migration in a Floating Paper Chip

Paula de Dios Andres, Brigitte Städler*

*Corresponding author for this work

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperJournal articleResearchpeer-review

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Abstract

In vitro epidermis models are important to evaluate and study disease progression and possible dermal drug delivery. An in vitro epidermis model using floating paper chips as a scaffold for proliferation and differentiation of primary human keratinocytes is reported. The formation of the four main layers of the epidermis (i.e., basal, spinosum, granulose, and cornified layers) is confirmed. The development of a cornified layer and the tight junction formation are evaluated as well as the alterations of organelles during the differentiation process. Further, this in vitro model is used to assess keratinocyte migration. Finally, magnetic micromotors are assembled, and their ability to aid cell migration on paper chips is confirmed when a static magnetic field is present. Taken together, this attempt to combine bottom-up synthetic biology with dermatology offers interesting opportunities for studying skin disease pathologies and evaluate possible treatments.

Original languageEnglish
Article number2201251
JournalSmall
Volume19
Issue13
Number of pages11
ISSN1613-6810
DOIs
Publication statusPublished - Mar 2023

Keywords

  • air–liquid interfaces
  • cell migration
  • keratinocyte differentiation
  • micromotors
  • paper chips
  • Humans
  • Keratinocytes
  • Epidermal Cells
  • Epidermis
  • Skin
  • Cell Differentiation
  • Cell Movement

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