Microfluidics-mediated isothermal detection of enzyme activity at the single molecule level

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Conventional analysis of enzymatic activity, often carried out on pools of cells, is blind to heterogeneity in the population. Here, we combine microfluidics with a previously developed isothermal rolling circle amplification-based assay to investigate multiple enzymatic activities in down to single cells. This microfluidics-meditated assay performs at very high sensitivity in picoliter incubators with small quantities of biological materials. Furthermore, we demonstrate the assay's capability of multiplexed detection of at least three enzyme activities at the single molecule level.
Original languageEnglish
JournalI E E E Engineering in Medicine and Biology Society. Conference Proceedings
Pages (from-to)3258-3261
Number of pages4
Publication statusPublished - 2011
Event2011 Annual International Conference of the IEEE - Boston, United States
Duration: 30 Aug 20113 Sep 2011


Conference2011 Annual International Conference of the IEEE
CountryUnited States

    Research areas

  • Cell Line, Enzymes, Humans, Microfluidics

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