Microfluidics-mediated isothermal detection of enzyme activity at the single molecule level

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaperConference articleResearchpeer-review

Conventional analysis of enzymatic activity, often carried out on pools of cells, is blind to heterogeneity in the population. Here, we combine microfluidics with a previously developed isothermal rolling circle amplification-based assay to investigate multiple enzymatic activities in down to single cells. This microfluidics-meditated assay performs at very high sensitivity in picoliter incubators with small quantities of biological materials. Furthermore, we demonstrate the assay's capability of multiplexed detection of at least three enzyme activities at the single molecule level.
Original languageEnglish
JournalI E E E Engineering in Medicine and Biology Society. Conference Proceedings
Volume2011
Pages (from-to)3258-3261
Number of pages4
ISSN2375-7477
DOIs
Publication statusPublished - 2011
Event2011 Annual International Conference of the IEEE - Boston, United States
Duration: 30 Aug 20113 Sep 2011

Conference

Conference2011 Annual International Conference of the IEEE
CountryUnited States
CityBoston
Period30/08/201103/09/2011

    Research areas

  • Cell Line, Enzymes, Humans, Microfluidics

See relations at Aarhus University Citationformats

ID: 48401003