Microfluidics and the quantification of biomolecular interactions

Daniel E Otzen, Alexander K. Buell, Henrik Jensen

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Abstract

Microfluidic systems under laminar flow conditions provide in-solution information about species size and binding affinities at very modest sample costs. Flow-induced dispersion analysis directly measures the spread of the analyte profile using Taylor dispersion analysis, whereas microfluidic diffusional sizing quantifies the transfer of analyte from one phase to another. Species of sizes between 0.5 and 1000 nm can be analyzed, and different populations resolved. Both techniques also allow analysis in complex media and medium throughput analysis. These properties make them valuable complements to existing approaches to measure biomolecular interactions.

Original languageEnglish
JournalCurrent Opinion in Structural Biology
Volume70
Pages (from-to)8-15
Number of pages8
ISSN0959-440X
DOIs
Publication statusPublished - Oct 2021

Keywords

  • Binding affinity
  • Flow-induced dispersion analysis
  • Hydrodynamic radius
  • Microfluidic diffusional sizing
  • Taylor dispersion analysis

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