Microfluidics and the quantification of biomolecular interactions

Daniel E Otzen; Alexander K. Buell; Henrik Jensen

doi:10.1016/j.sbi.2021.02.006

Microfluidics and the quantification of biomolecular interactions

Daniel E Otzen, Alexander K. Buell, Henrik Jensen

Research output: Contribution to journal/Conference contribution in journal/Contribution to newspaper › Journal article › Research › peer-review

18 Citations (Scopus)

92 Downloads (Pure)

Abstract

Microfluidic systems under laminar flow conditions provide in-solution information about species size and binding affinities at very modest sample costs. Flow-induced dispersion analysis directly measures the spread of the analyte profile using Taylor dispersion analysis, whereas microfluidic diffusional sizing quantifies the transfer of analyte from one phase to another. Species of sizes between 0.5 and 1000 nm can be analyzed, and different populations resolved. Both techniques also allow analysis in complex media and medium throughput analysis. These properties make them valuable complements to existing approaches to measure biomolecular interactions.

Original language	English
Journal	Current Opinion in Structural Biology
Volume	70
Pages (from-to)	8-15
Number of pages	8
ISSN	0959-440X
DOIs	https://doi.org/10.1016/j.sbi.2021.02.006
Publication status	Published - Oct 2021

Keywords

Binding affinity
Flow-induced dispersion analysis
Hydrodynamic radius
Microfluidic diffusional sizing
Taylor dispersion analysis

Access to Document

10.1016/j.sbi.2021.02.006Licence: CC BY

1-s2.0-S0959440X21000294-mainFinal published version, 1.63 MBLicence: CC BY

Cite this

@article{f1281ea892274452ba4395ac0b27afb8,

title = "Microfluidics and the quantification of biomolecular interactions",

abstract = "Microfluidic systems under laminar flow conditions provide in-solution information about species size and binding affinities at very modest sample costs. Flow-induced dispersion analysis directly measures the spread of the analyte profile using Taylor dispersion analysis, whereas microfluidic diffusional sizing quantifies the transfer of analyte from one phase to another. Species of sizes between 0.5 and 1000 nm can be analyzed, and different populations resolved. Both techniques also allow analysis in complex media and medium throughput analysis. These properties make them valuable complements to existing approaches to measure biomolecular interactions.",

keywords = "Binding affinity, Flow-induced dispersion analysis, Hydrodynamic radius, Microfluidic diffusional sizing, Taylor dispersion analysis",

author = "Otzen, {Daniel E} and Buell, {Alexander K.} and Henrik Jensen",

year = "2021",

month = oct,

doi = "10.1016/j.sbi.2021.02.006",

language = "English",

volume = "70",

pages = "8--15",

journal = "Current Opinion in Structural Biology",

issn = "0959-440X",

publisher = "Elsevier Ltd",

}

TY - JOUR

T1 - Microfluidics and the quantification of biomolecular interactions

AU - Otzen, Daniel E

AU - Buell, Alexander K.

AU - Jensen, Henrik

PY - 2021/10

Y1 - 2021/10

N2 - Microfluidic systems under laminar flow conditions provide in-solution information about species size and binding affinities at very modest sample costs. Flow-induced dispersion analysis directly measures the spread of the analyte profile using Taylor dispersion analysis, whereas microfluidic diffusional sizing quantifies the transfer of analyte from one phase to another. Species of sizes between 0.5 and 1000 nm can be analyzed, and different populations resolved. Both techniques also allow analysis in complex media and medium throughput analysis. These properties make them valuable complements to existing approaches to measure biomolecular interactions.

AB - Microfluidic systems under laminar flow conditions provide in-solution information about species size and binding affinities at very modest sample costs. Flow-induced dispersion analysis directly measures the spread of the analyte profile using Taylor dispersion analysis, whereas microfluidic diffusional sizing quantifies the transfer of analyte from one phase to another. Species of sizes between 0.5 and 1000 nm can be analyzed, and different populations resolved. Both techniques also allow analysis in complex media and medium throughput analysis. These properties make them valuable complements to existing approaches to measure biomolecular interactions.

KW - Binding affinity

KW - Flow-induced dispersion analysis

KW - Hydrodynamic radius

KW - Microfluidic diffusional sizing

KW - Taylor dispersion analysis

UR - http://www.scopus.com/inward/record.url?scp=85103691288&partnerID=8YFLogxK

U2 - 10.1016/j.sbi.2021.02.006

DO - 10.1016/j.sbi.2021.02.006

M3 - Journal article

C2 - 33831785

SN - 0959-440X

VL - 70

SP - 8

EP - 15

JO - Current Opinion in Structural Biology

JF - Current Opinion in Structural Biology

ER -

Microfluidics and the quantification of biomolecular interactions

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this