Abstract
Aim
The chorioallantoic membrane (CAM) model is a scalable preclinical model capable of high-throughput tumor-bearing in vivo growth of tumor material for oncological research. Our purpose has been to establish the necessary methods for the CAM model to become an attractive platform for advanced nuclear medicine and proton radiation experiments on CAM tumors. The model is based on an extra-embryonal membrane in the eggs of avian and reptilian species.
Methods & Results
Commercial Dekalb White eggs (gallus gallus domesticus) were opened in a square window and grafted with C3H mammary carcinoma or MOC2 mouse oral carcinoma tumor pieces. We established feasibility of in ovo proton irradiation and optical coherence tomography (OCT) of tumor vasculature. Radioactive tracer uptake via alternative administration routes was proved and quitified with PET and autoradiography.
Conclusions
Autoradiography was performed on 53 tumors and PET scans of 11 tumors were obtained after using alternative administration routes of various tracers to evaluate intratumoral distribution and pharmacokinetics. Furthermore, we have investigated in ovo proton irradiation and following growth on 16 tumors. To evaluate vessel morphology, we have developed a tool using OCT angiography data to investigate key vessel characteristics. The tumor-bearing CAM model is highly relevant as a preclinical model in nuclear medicine and radiation biology. In ovo proton irradiation with OCT angiography follow-up allows for high radiation doses and functional, non-invasive post-irradiation vascular evaluation. Autoradiography and PET scans after yolk injection of tracers can be used to evaluate physiological changes of vascularization and tumor micromilleu post-irradiation.
The chorioallantoic membrane (CAM) model is a scalable preclinical model capable of high-throughput tumor-bearing in vivo growth of tumor material for oncological research. Our purpose has been to establish the necessary methods for the CAM model to become an attractive platform for advanced nuclear medicine and proton radiation experiments on CAM tumors. The model is based on an extra-embryonal membrane in the eggs of avian and reptilian species.
Methods & Results
Commercial Dekalb White eggs (gallus gallus domesticus) were opened in a square window and grafted with C3H mammary carcinoma or MOC2 mouse oral carcinoma tumor pieces. We established feasibility of in ovo proton irradiation and optical coherence tomography (OCT) of tumor vasculature. Radioactive tracer uptake via alternative administration routes was proved and quitified with PET and autoradiography.
Conclusions
Autoradiography was performed on 53 tumors and PET scans of 11 tumors were obtained after using alternative administration routes of various tracers to evaluate intratumoral distribution and pharmacokinetics. Furthermore, we have investigated in ovo proton irradiation and following growth on 16 tumors. To evaluate vessel morphology, we have developed a tool using OCT angiography data to investigate key vessel characteristics. The tumor-bearing CAM model is highly relevant as a preclinical model in nuclear medicine and radiation biology. In ovo proton irradiation with OCT angiography follow-up allows for high radiation doses and functional, non-invasive post-irradiation vascular evaluation. Autoradiography and PET scans after yolk injection of tracers can be used to evaluate physiological changes of vascularization and tumor micromilleu post-irradiation.
| Translated title of the contribution | Metodologiske fremskridt i CAM-modellen: Æggeblommeleverede tracere, dobbeltsporingsautoradiografi, dynamisk PET, in ovo protonbestråling og OCT til vaskulær vurdering |
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| Original language | English |
| Publication date | 11 Sept 2025 |
| Publication status | Published - 11 Sept 2025 |
| Event | 40th congress of the international union of physiological sciences - Frankfurt, Germany Duration: 11 Sept 2025 → 14 Sept 2025 https://www.iups2025.com |
Conference
| Conference | 40th congress of the international union of physiological sciences |
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| Location | Frankfurt |
| Country/Territory | Germany |
| Period | 11/09/2025 → 14/09/2025 |
| Internet address |