Metabolism of acyclic and cyclic N-nitroamines by cultured human colon

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  • Herman Autrup, Human Tissue Studies Section, Experimental Pathology Branch, Division of Cancer Cause and Prevention, NCI, National Institutes of Health, Public Health Service, U.S. Department of Health, Education, and Welfare, Bethesda, Maryland, Denmark
  • Curtis C. Harris, United States
  • Benjamin F. Trump, United States
  • Institute of Environmental and Occupational Medicine
Cultured human colon mucosa was found to metabolize both acyclic and cyclic N-nitrosamines as measured by 14C-CO2 formation and reaction of the activated moieties with cellular macromolecules. Dimethylnitrosamine and N-nitrosopyrrolidine were metabolized by explants from all patients studied. A positive correlation between binding of dimethylnitrosamine to DNA and CO2-formation was observed. DMN alkylated DNA in both O-6 and N-7 position of guanine. However, most of the radioactivity was associated with an acid labile compound. High binding levels of N,N'-dinitrosopiperazine to protein without concomitant binding to DNA were detected. Inter-individual variation in both binding level to DNA and ability to metabolize the different N-nitrosamines was observed.

We would like to thank Drs. U. Saffiotti, G. Stoner, and T. Bowden for valuable comments, Ms. R. Schwartz for technical assistance, and Mrs. M. Bellman for secretarial assistance.
Original languageEnglish
JournalExperimental Biology and Medicine
Pages (from-to)111-115
Number of pages5
Publication statusPublished - Oct 1978

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