Meta-analysis of cable bacteria and their associates in existing next-generation sequencing datasets: cables are everywhere

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Meta-analysis of cable bacteria and their associates in existing next-generation sequencing datasets: cables are everywhere. / Marshall, Ian P.G.; Burdorf, Laurine D. W.; Schramm, Andreas.

2019. Poster session presented at Electromicrobiology 2019, Aarhus, Denmark.

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@conference{aba78fe07b244386b9ec4a080764feeb,
title = "Meta-analysis of cable bacteria and their associates in existing next-generation sequencing datasets: cables are everywhere",
abstract = "The sediment environments inhabited by cable bacteria have been the subject ofinvestigations by microbial ecologists for decades, but it is only in the last few years that we have become aware of cable bacteria, particularly the uncultivated candidate genera Electrothrix and Electronema. There are many publicly available datasets from next-generation sequencing of 16S rRNA gene amplicons from environmental samples where cablebacteria sequences may be present, but were overlooked in the initial analysis. We have begun to re-examine these existing datasets, alongside some of our own new datasets, to determine the extent of cable bacteria diversity, and to identify patterns in their distribution to aid our understanding of cable bacteria ecology. Moreover, we aim to identify other microbial taxa that co-occur with cable bacteria, possibly indicating an association between cable bacteria and members of the surrounding microbial community. We have scanned 259,144 existingsamples in the Sequence Read Archive (SRA), and identified cable bacteria in 4,259 of these. These samples contained up to 10.7{\%} Electrothrix/Electronema. We have used these datasets to obtain a comprehensive picture of the diversity within the candidate genera Electrothrix and Electronema, and to identify putative microbes that live in association with cable bacteria.",
author = "Marshall, {Ian P.G.} and Burdorf, {Laurine D. W.} and Andreas Schramm",
year = "2019",
language = "English",
note = "Electromicrobiology 2019 ; Conference date: 21-03-2019 Through 22-03-2019",

}

RIS

TY - CONF

T1 - Meta-analysis of cable bacteria and their associates in existing next-generation sequencing datasets: cables are everywhere

AU - Marshall, Ian P.G.

AU - Burdorf, Laurine D. W.

AU - Schramm, Andreas

PY - 2019

Y1 - 2019

N2 - The sediment environments inhabited by cable bacteria have been the subject ofinvestigations by microbial ecologists for decades, but it is only in the last few years that we have become aware of cable bacteria, particularly the uncultivated candidate genera Electrothrix and Electronema. There are many publicly available datasets from next-generation sequencing of 16S rRNA gene amplicons from environmental samples where cablebacteria sequences may be present, but were overlooked in the initial analysis. We have begun to re-examine these existing datasets, alongside some of our own new datasets, to determine the extent of cable bacteria diversity, and to identify patterns in their distribution to aid our understanding of cable bacteria ecology. Moreover, we aim to identify other microbial taxa that co-occur with cable bacteria, possibly indicating an association between cable bacteria and members of the surrounding microbial community. We have scanned 259,144 existingsamples in the Sequence Read Archive (SRA), and identified cable bacteria in 4,259 of these. These samples contained up to 10.7% Electrothrix/Electronema. We have used these datasets to obtain a comprehensive picture of the diversity within the candidate genera Electrothrix and Electronema, and to identify putative microbes that live in association with cable bacteria.

AB - The sediment environments inhabited by cable bacteria have been the subject ofinvestigations by microbial ecologists for decades, but it is only in the last few years that we have become aware of cable bacteria, particularly the uncultivated candidate genera Electrothrix and Electronema. There are many publicly available datasets from next-generation sequencing of 16S rRNA gene amplicons from environmental samples where cablebacteria sequences may be present, but were overlooked in the initial analysis. We have begun to re-examine these existing datasets, alongside some of our own new datasets, to determine the extent of cable bacteria diversity, and to identify patterns in their distribution to aid our understanding of cable bacteria ecology. Moreover, we aim to identify other microbial taxa that co-occur with cable bacteria, possibly indicating an association between cable bacteria and members of the surrounding microbial community. We have scanned 259,144 existingsamples in the Sequence Read Archive (SRA), and identified cable bacteria in 4,259 of these. These samples contained up to 10.7% Electrothrix/Electronema. We have used these datasets to obtain a comprehensive picture of the diversity within the candidate genera Electrothrix and Electronema, and to identify putative microbes that live in association with cable bacteria.

M3 - Poster

ER -